Mouse anti-Human PSMD10/Gankyrin Monoclonal Antibody | anti-PSMD10 antibody

Anti-PSMD10/Gankyrin Antibody (clone 4B5)

Cat. Num. AAA2400906

• Gene Names: PSMD10; p28; p28(GANK); dJ889N15.2
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified from ascites by Protein A
• Synonyms: PSMD10/Gankyrin; Monoclonal Antibody; Anti-PSMD10/Gankyrin Antibody (clone 4B5); Mouse Monoclonal [clone 3F6] (IgG1) to Human PSMD10/Gankyrin; PSMD10; 26s proteasome subunit 10; Ankyrin repeat protein; DJ889N15.2; Gankyrin; p28; p28(GANK); anti-PSMD10 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, k
• Clone Number: 4B5
• Purity/Purification: Purified from ascites by Protein A
• Form/Format: PBS, pH 7.4.
• Sequence Length: 226

• Applicable Applications for anti-PSMD10 antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin, Western Blot (WB)
• Application Notes: IF: 10 ug/ml; IHC-Fr: 1:50
• Immunogen: PSMD10 (AAH11960, 127 a.a. ~ 226 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.; Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(IHC with PSMD10 antibody on frozen human tonsil.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to PSMD10 on HeLa cell (antibody concentration 10 ug/ml).)

Western Blot (WB)

(PSMD10 monoclonal antibody (M01), clone 4B5 Western blot of PSMD10 expression in HeLa.)

ELISA (EIA)

(Detection limit for recombinant GST tagged PSMD10 is approximately 0.3 ng/ml as a capture antibody.)

Related Product Information for anti-PSMD10 antibody

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pahway, leading to prolonged AKT activation.

Product Categories/Family for anti-PSMD10 antibody

Protease Non Catalytic

NCBI and Uniprot Product Information

• NCBI GI #: 4506217
• NCBI GeneID: 5716
• NCBI Accession #: NP_002805.1
• NCBI GenBank Nucleotide #: NM_002814.3
• UniProt Accession #: O75832; Q5U0B2; Q8IZK9
• Molecular Weight: 16,137 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 10 isoform 1
• NCBI Official Synonym Full Names: proteasome 26S subunit, non-ATPase 10
• NCBI Official Symbol: PSMD10
• NCBI Official Synonym Symbols: p28; p28(GANK); dJ889N15.2
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 10
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 10
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD10

NCBI Description

This gene encodes a subunit of the PA700/19S complex, which is the regulatory component of the 26S proteasome. The 26S proteosome complex is required for ubiquitin-dependent protein degradation. This protein is a non-ATPase subunit that may be involved in protein-protein interactions. Aberrant expression of this gene may paly a role in tumorigenesis. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.[provided by RefSeq, Mar 2011]

Uniprot Description

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pathway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis.Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export.CautionWas initially identified as a genuine component of the 26S proteasome.