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Testing Data

Rabbit anti-Rat Cytochrome c Polyclonal Antibody | anti-Cycs antibody

Cytochrome c Antibody

Gene Names
Cycs; CYCSA
Reactivity
Rat
Applications
Western Blot
Purity
Affinity purified
Synonyms
Cytochrome c; Polyclonal Antibody; Cytochrome c Antibody; CYCS; CYC; anti-Cycs antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
100 ug (0.2 mg/ml) affinity purified rabbit anti-Cytochrome c polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Appearance: Colorless liquid
Concentration
0.2 mg/ml (varies by lot)
Sequence Length
105
Applicable Applications for anti-Cycs antibody
Western Blot (WB)
Application Notes
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Rat kidney lysate can be used as a positive control. The cytochrome c antibody detects the 12 kDa cytochrome c from rat samples.
Immunogen
Rat cytochrome c
Handling
The antibody solution should be gently mixed before use.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months

Testing Data

Testing Data
Related Product Information for anti-Cycs antibody
Background: Cytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,605 Da
NCBI Official Full Name
cytochrome c, somatic
NCBI Official Synonym Full Names
cytochrome c, somatic
NCBI Official Symbol
Cycs
NCBI Official Synonym Symbols
CYCSA
NCBI Protein Information
cytochrome c, somatic
UniProt Protein Name
Cytochrome c, somatic
Protein Family
UniProt Gene Name
Cycs
UniProt Entry Name
CYC_RAT

NCBI Description

a component of the electron transport chain in mitochondria, may function in apoptosis [RGD, Feb 2006]

Uniprot Description

CYCS: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Belongs to the cytochrome c family.

Protein type: Oxidoreductase; Protein phosphatase, Ser/Thr (non-receptor); Mitochondrial; Apoptosis

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial intermembrane space; cytosol; nucleus; mitochondrial respiratory chain

Molecular Function: electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity; electron carrier activity; iron ion binding; heme binding; lipid binding

Biological Process: apoptosis; hydrogen peroxide metabolic process; mitochondrial electron transport, cytochrome c to oxygen; DNA fragmentation during apoptosis; caspase activation via cytochrome c; response to oxidative stress; mitochondrial electron transport, ubiquinol to cytochrome c

Research Articles on Cycs

Similar Products

Product Notes

The Cycs cycs (Catalog #AAA841135) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cytochrome c Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome c can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Rat kidney lysate can be used as a positive control. The cytochrome c antibody detects the 12 kDa cytochrome c from rat samples. Researchers should empirically determine the suitability of the Cycs cycs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome c, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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