Typical Testing Data/Standard Curve (for reference only)
Mouse Prostasin ELISA Kit | PRSS8 elisa kit
Mouse Prostasin PicoKine ELISA Kit
Gene Names
Prss8; fr; CAP1; mCAP1; C79772; AI313909; 2410039E18Rik
Reactivity
Mouse
Synonyms
Prostasin; Mouse Prostasin PicoKine ELISA Kit; protease; serine; 8; 2410039E18Rik; AI313909; C79772; CAP1; Channel Activating Protease 1; mCAP1; Prostasin heavy chain; Prss8; PRSS8_HUMAN; Serine protease 8; PRSS8 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse Prostasin
Sequence Length
342
Samples
Cell culture supernates, serum, plasma (heparin, EDTA) and urine
Assay Type
Sandwich
Detection Range
0.78ng/ml-50ng/ml
Sensitivity
< 50pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PRSS8 elisa kit
Principle of the assay: mouse Prostasin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Prostasin has been precoated onto 96-well plates. Standards(NSO, A30-Q289) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Prostasin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Prostasin amount of sample captured in plate.
Background: Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60% to 80% increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.
Background: Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60% to 80% increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
36,729 Da
NCBI Official Full Name
Prostasin
NCBI Official Synonym Full Names
protease, serine 8 (prostasin)
NCBI Official Symbol
Prss8
NCBI Official Synonym Symbols
fr; CAP1; mCAP1; C79772; AI313909; 2410039E18Rik
NCBI Protein Information
prostasin; serine protease 8; channel activating protease 1; channel-activating protease 1; protease, serine, 8 (prostasin)
UniProt Protein Name
Prostasin
Protein Family
UniProt Gene Name
Prss8
UniProt Synonym Gene Names
Cap1; CAP1
UniProt Entry Name
PRSS8_MOUSE
Uniprot Description
PRSS8: Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates. Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G). Belongs to the peptidase S1 family.
Protein type: Membrane protein, integral; EC 3.4.21.-; Protease
Cellular Component: extrinsic to plasma membrane; extracellular space; anchored to plasma membrane; membrane; extracellular region; integral to membrane; plasma membrane; lipid raft
Molecular Function: peptidase activity; sodium channel regulator activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity
Biological Process: hair follicle development; proteolysis
Research Articles on PRSS8
Similar Products
Product Notes
The Mouse PRSS8 prss8 (Catalog #AAA177224) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177224 ELISA Kit recognizes Mouse PRSS8. It is sometimes possible for the material contained within the vial of "Prostasin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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