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Western Blot (WB) (Western blot analysis using BMI1 mAb against human BMI1 (AA)

Mouse anti-Human BMI1 Monoclonal Antibody | anti-BMI1 antibody

Anti-BMI1 Mouse mAb

Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Synonyms
BMI1; Monoclonal Antibody; Anti-BMI1 Mouse mAb; PCGF4; RNF51; MGC12685; FLVI2/BMI1; anti-BMI1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3E3
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
326
Applicable Applications for anti-BMI1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Immunogen
Purified recombinant fragment of human BMI1 expressed in E Coli.

Western Blot (WB)

(Western blot analysis using BMI1 mAb against human BMI1 (AA)

Western Blot (WB) (Western blot analysis using BMI1 mAb against human BMI1 (AA)

Western Blot (WB)

(Western blot analysis using BMI1 mAb against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.)

Western Blot (WB) (Western blot analysis using BMI1 mAb against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.)

Immunofluorescence (IF)

(Immunofluorescence analysis of Hela cells using BMI1 mouse mAb (green). Blue)

Immunofluorescence (IF) (Immunofluorescence analysis of Hela cells using BMI1 mouse mAb (green). Blue)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NIH/3T3 cells using BMI1 mouse mAb (green) and negative control (red).)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of NIH/3T3 cells using BMI1 mouse mAb (green) and negative control (red).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using BMI1 mouse mAb with DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using BMI1 mouse mAb with DAB staining.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using BMI1 mouse mAb with DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using BMI1 mouse mAb with DAB staining.)
Related Product Information for anti-BMI1 antibody
Entrez Summary: Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37
NCBI Official Full Name
BMI1 polycomb ring finger oncogene
NCBI Official Symbol
BMI1
NCBI Protein Information
BMI1 polycomb ring finger oncogene
Protein Family

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Product Notes

The BMI1 (Catalog #AAA476229) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-BMI1 Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BMI1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the BMI1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BMI1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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