Human Prostasin ELISA Kit | PRSS8 elisa kit

Human Prostasin PicoKine ELISA Kit

Cat. Num. AAA177380

• Gene Names: PRSS8; CAP1; PROSTASIN
• Reactivity: Human
• Synonyms: Prostasin; Human Prostasin PicoKine ELISA Kit; protease; serine; 8; 2410039E18Rik; AI313909; C79772; CAP1; Channel Activating Protease 1; mCAP1; Prostasin heavy chain; Prss8; PRSS8_HUMAN; Serine protease 8; PRSS8 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human Prostasin
• Sequence Length: 343

• Assay Type: Quantitative Sandwich
• Detection Range: 1.56ng/ml-100ng/ml
• Sensitivity: <50pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRSS8 elisa kit

Principle of the Assay: Human Prostasin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from sheep specific for Prostasin has been precoated onto 96-well plates. Standards(Expression system for standard: CHO; Immunogen sequence: A33-G319) and test samples are added to the wells, a biotinylated detection polyclonal antibody from sheep specific for Prostasin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Prostasin amount of sample captured in plate.

Background: Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60% to 80% increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.

NCBI and Uniprot Product Information

• NCBI GI #: 2833277
• NCBI GeneID: 5652
• UniProt Accession #: Q16651; Q9UCA3; B4DWP2
• Molecular Weight: 30,519 Da
• NCBI Official Full Name: Prostasin
• NCBI Official Synonym Full Names: protease, serine, 8
• NCBI Official Symbol: PRSS8
• NCBI Official Synonym Symbols: CAP1; PROSTASIN
• NCBI Protein Information: prostasin; serine protease 8; channel-activating protease 1
• UniProt Protein Name: Prostasin
• Protein Family: Prostasin
• UniProt Gene Name: PRSS8
• UniProt Synonym Gene Names: CAP1
• UniProt Entry Name: PRSS8_HUMAN

NCBI Description

This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine. [provided by RefSeq, Jul 2008]

Uniprot Description

PRSS8: Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates. Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G). Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.-; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: extracellular space; plasma membrane; extracellular region; integral to membrane

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: proteolysis