Typical Testing Data/Standard Curve (for reference only)
(Mouse Marapsin/Pancresin PicoKine ELISA Kit standard curve )
Mouse Marapsin/Pancresin ELISA Kit | PRSS27 elisa kit
Mouse Marapsin/Pancresin PicoKine ELISA Kit
Gene Names
Prss27; Mpn; CAPH2; marapsin
Reactivity
Mouse
Synonyms
Marapsin/Pancresin; Mouse Marapsin/Pancresin PicoKine ELISA Kit; CAPH2; Marapsin; MPN; Pancreasin; Prss27; Prss 27; Protease serine 27; UNQ1884/PRO4327; Q8BJR6; Serine protease 27; protease; serine 27; PRSS27 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Marapsin/Pancresin
Sequence Length
328
Samples
Cell culture supernates and serum.
Assay Type
Sandwich
Detection Range
125pg/ml-8000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
Typical Testing Data/Standard Curve (for reference only)
(Mouse Marapsin/Pancresin PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse Marapsin/Pancresin PicoKine ELISA Kit standard curve )
Related Product Information for PRSS27 elisa kit
Principle of the Assay: Mouse Marapsin/Pancresin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Marapsin/Pancresin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: M38-V328) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Marapsin/Pancresin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Marapsin/Pancresin amount of sample captured in plate.
Background/Introduction: Pancreasin, also known as PRSS27 or Marapsin, is a pancreatic tryptic serine peptidase that cleaves peptides after an arginine residue. It belongs to the group-1 subfamily of serine proteases. The pancreasin gene is mapped to chromosome 16p13.3. Pancreasin is resistant to inactivation by large molecular mass trypsin inhibitors, but it is sensitive to benzamidine and leupeptin.
Background/Introduction: Pancreasin, also known as PRSS27 or Marapsin, is a pancreatic tryptic serine peptidase that cleaves peptides after an arginine residue. It belongs to the group-1 subfamily of serine proteases. The pancreasin gene is mapped to chromosome 16p13.3. Pancreasin is resistant to inactivation by large molecular mass trypsin inhibitors, but it is sensitive to benzamidine and leupeptin.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,789 Da
NCBI Official Full Name
serine protease 27
NCBI Official Synonym Full Names
protease, serine 27
NCBI Official Symbol
Prss27
NCBI Official Synonym Symbols
Mpn; CAPH2; marapsin
NCBI Protein Information
serine protease 27
UniProt Protein Name
Serine protease 27
Protein Family
UniProt Gene Name
Prss27
UniProt Synonym Gene Names
Mpn
UniProt Entry Name
PRS27_MOUSE
Uniprot Description
PRSS27: Belongs to the peptidase S1 family
Protein type: Protease; EC 3.4.21.-; Secreted, signal peptide; Secreted
Cellular Component: plasma membrane
Research Articles on PRSS27
Similar Products
Product Notes
The Mouse PRSS27 prss27 (Catalog #AAA178538) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178538 ELISA Kit recognizes Mouse PRSS27. It is sometimes possible for the material contained within the vial of "Marapsin/Pancresin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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