Typical Testing Data/Standard Curve (for reference only)
(Mouse NOPE/IGDCC4 PicoKine ELISA Kit standard curve )
Mouse NOPE/IGDCC4 ELISA Kit | IGDCC4 elisa kit
Mouse NOPE/IGDCC4 PicoKine ELISA Kit
Gene Names
Igdcc4; Nope; DDM36
Reactivity
Mouse
Synonyms
NOPE/IGDCC4; Mouse NOPE/IGDCC4 PicoKine ELISA Kit; DDM 36; DDM36; hDDM 36; hDDM36; IGDCC4; KIAA1628; NOPE; Q9EQS9; Immunoglobulin superfamily DCC subclass member 4; immunoglobulin superfamily DCC subclass member 4; IGDCC4 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse NOPE/IGDCC4
Sequence Length
1253
Samples
Cell culture supernates, serum and plasma(heparin, EDTA).
Assay Type
Sandwich
Detection Range
125pg/ml-8000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
Typical Testing Data/Standard Curve (for reference only)
(Mouse NOPE/IGDCC4 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse NOPE/IGDCC4 PicoKine ELISA Kit standard curve )
Related Product Information for IGDCC4 elisa kit
Principle of the Assay: Mouse NOPE/IGDCC4 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for NOPE/IGDCC4 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: E25-H954) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NOPE/IGDCC4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse NOPE/IGDCC4 amount of sample captured in plate.
Background/Introduction: IGDCC4, also known as NOPE, plays essential roles in biologic functions of the cell surface, including cell adhesion, cell migration, and signal transduction. NOPE is identified as an oncofetal marker of murine and human hepatocellular carcinoma (HCC). Its expression is elevated in mouse and human hepatoma cell lines and in mouse hepatomas, but it is not expressed in normal liver or preneoplastic mouse HCC. Furthermore, NOPE is expressed in both AFP -positive and -negative tumors and is specifically expressed in epithelial tumor cells.
Background/Introduction: IGDCC4, also known as NOPE, plays essential roles in biologic functions of the cell surface, including cell adhesion, cell migration, and signal transduction. NOPE is identified as an oncofetal marker of murine and human hepatocellular carcinoma (HCC). Its expression is elevated in mouse and human hepatoma cell lines and in mouse hepatomas, but it is not expressed in normal liver or preneoplastic mouse HCC. Furthermore, NOPE is expressed in both AFP -positive and -negative tumors and is specifically expressed in epithelial tumor cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
139,524 Da
NCBI Official Full Name
immunoglobulin superfamily DCC subclass member 4 isoform 1
NCBI Official Synonym Full Names
immunoglobulin superfamily, DCC subclass, member 4
NCBI Official Symbol
Igdcc4
NCBI Official Synonym Symbols
Nope; DDM36
NCBI Protein Information
immunoglobulin superfamily DCC subclass member 4
UniProt Protein Name
Immunoglobulin superfamily DCC subclass member 4
Protein Family
UniProt Gene Name
Igdcc4
UniProt Synonym Gene Names
Ddm36; Kiaa1628; Nope
UniProt Entry Name
IGDC4_MOUSE
Uniprot Description
NOPE: Belongs to the immunoglobulin superfamily. DCC family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: integral to plasma membrane
Research Articles on IGDCC4
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Product Notes
The Mouse IGDCC4 igdcc4 (Catalog #AAA178558) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178558 ELISA Kit recognizes Mouse IGDCC4. It is sometimes possible for the material contained within the vial of "NOPE/IGDCC4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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