Western Blot (WB)
(Anti-PRSS27 rabbit monoclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:32 kDaObserved band size:32 kDa)
Rabbit anti-Human Marapsin Monoclonal Antibody | anti-PRSS27 antibody
Recombinant Anti-Marapsin Antibody, Rabbit Monoclonal
Gene Names
PRSS27; MPN; CAPH2
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A
Synonyms
Marapsin; Monoclonal Antibody; Recombinant Anti-Marapsin Antibody; Rabbit Monoclonal; Pancreasin/Marapsin/PRSS27 Antibody; Rabbit MAb; protease; serine 27; Anti-CAPH2 Antibody; Anti-MPN Antibody; Anti-UNQ1884/PRO4327 Antibody; anti-PRSS27 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
107
Specificity
Human Pancreasin/Marapsin/PRSS27
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PRSS27 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IP: 4-6uL/mg of lysate
IP: 4-6uL/mg of lysate
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human Pancreasin/Marapsin/PRSS27 protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Pancreasin/Marapsin/PRSS27 (rh Pancreasin/Marapsin/PRSS27).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PRSS27 rabbit monoclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:32 kDaObserved band size:32 kDa)
Western Blot (WB)
(Anti-PRSS27 rabbit monoclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:32 kDaObserved band size:32 kDa)
Immunoprecipitation (IP)
(PRSS27 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate2 uL anti-PRSS27 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PRSS27 rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 32 kDaObserved band size: 32 kDa)
Immunoprecipitation (IP)
(PRSS27 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate2 uL anti-PRSS27 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PRSS27 rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 32 kDaObserved band size: 32 kDa)
Related Product Information for anti-PRSS27 antibody
The name "Pancreasin" because it is transcribed strongly in the pancreas. This secreted, tryptic serine protease, also known as Marapsin or PRSS27 (Protease, serine, 27), is a member of the peptidase S1 family. Pancreasin is inhibited by benzamidine and leupeptin but resists several classic inhibitors of trypsin. Marapsin was constitutively expressed in nonkeratinizing stratified squamous epithelia of human esophagus, tonsil, cervix, larynx, and cornea. In fact, marapsin was the second most strongly up-regulated protease in psoriatic lesions, where expression was localized to the upper region of the hyperplastic epidermis. Similarly, in the hyperproliferative epithelium of regenerating murine skin wounds, marapsin localized to the suprabasal layers, where keratinocytes undergo squamous differentiation. Marapsin's restricted expression, localization, and cytokine-inducible expression suggest a role in the terminal differentiation of keratinocytes in hyperproliferating squamous epithelia.
Product Categories/Family for anti-PRSS27 antibody
References
Bhagwandin VJ, et al. (2003) Structure and activity of human pancreasin, a novel tryptic serine peptidase expressed primarily by the pancreas. J Biol Chem. 278(5): 3363-71.Li W, et al. (2009) The serine protease marapsin is expressed in stratified squamous epithelia and is up-regulated in the hyperproliferative epidermis of psoriasis and regenerating wounds. J Biol Chem. 284(1): 218-28.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31,940 Da
NCBI Official Full Name
serine protease 27 isoform 2
NCBI Official Synonym Full Names
protease, serine 27
NCBI Official Symbol
PRSS27
NCBI Official Synonym Symbols
MPN; CAPH2
NCBI Protein Information
serine protease 27
UniProt Protein Name
Serine protease 27
Protein Family
UniProt Gene Name
PRSS27
UniProt Synonym Gene Names
MPN
UniProt Entry Name
PRS27_HUMAN
NCBI Description
This gene is located within a large protease gene cluster on chromosome 16. It belongs to the group-1 subfamily of serine proteases. The encoded protein is a secreted tryptic serine protease and is expressed mainly in the pancreas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
Uniprot Description
PRSS27: Belongs to the peptidase S1 family
Protein type: EC 3.4.21.-; Secreted, signal peptide; Secreted; Protease
Chromosomal Location of Human Ortholog: 16p13.3
Research Articles on PRSS27
Similar Products
Product Notes
The PRSS27 prss27 (Catalog #AAA8106193) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-Marapsin Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Marapsin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 4-6uL/mg of lysate. Researchers should empirically determine the suitability of the PRSS27 prss27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Marapsin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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