Immunohistochemistry (IHC)
(Anti-WISP1 antibody, MBS176363, IHC(P)IHC(P): Rat Kidney Tissue )
Rabbit WISP1 Polyclonal Antibody | anti-WISP1 antibody
Anti-WISP1 antibody
Immunohistochemistry (IHC)
(Anti-WISP1 antibody, MBS176363, IHC(P)IHC(P): Rat Kidney Tissue )
Immunohistochemistry (IHC)
(Anti-WISP1 antibody, MBS176363, IHC(P)IHC(P): Rat Kidney Tissue )
Immunohistochemistry (IHC)
(Anti-WISP1 antibody, MBS176363, IHC(P)IHC(P): Rat Pancreas Tissue )
Immunohistochemistry (IHC)
(Anti-WISP1 antibody, MBS176363, IHC(P)IHC(P): Rat Pancreas Tissue )
Immunohistochemistry (IHC)
(Anti-WISP1 antibody, MBS176363, IHC(P)IHC(P): Human Placenta Tissue )
Immunohistochemistry (IHC)
(Anti-WISP1 antibody, MBS176363, IHC(P)IHC(P): Human Placenta Tissue )
Western Blot (WB)
(Anti-WISP1 antibody, MBS176363, Western blottingAll lanes: Anti WISP1 (MBS176363) at 0.5ug/mlLane 1: Rat Heart Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: Rat Lung Tissue Lysate at 50ugLane 4: A549 Whole Cell Lysate at 40ugLane 5: A431 Whole Cell Lysate at 40ugLane 6: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 40KDObserved bind size: 40KD)
Western Blot (WB)
(Anti-WISP1 antibody, MBS176363, Western blottingAll lanes: Anti WISP1 (MBS176363) at 0.5ug/mlLane 1: Rat Heart Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: Rat Lung Tissue Lysate at 50ugLane 4: A549 Whole Cell Lysate at 40ugLane 5: A431 Whole Cell Lysate at 40ugLane 6: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 40KDObserved bind size: 40KD)
Immunocytochemistry (ICC)
(Anti-WISP1 antibody, MBS176363, ICCICC: MCF-7 Cell )
Immunocytochemistry (ICC)
(Anti-WISP1 antibody, MBS176363, ICCICC: MCF-7 Cell )
Background: WISP1(WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed(Tanaka et al., 2001). By use of radiation hybrid mapping panels, Pennica et al.(1998) mapped the WISP1 to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. Pennica et al.(1998) found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84% of tumors examined compared with patient-matched normal mucosa.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2011]
Uniprot Description
WISP1: Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. Belongs to the CCN family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted; Oncoprotein
Chromosomal Location of Human Ortholog: 8q24.22
Cellular Component: extracellular space; proteinaceous extracellular matrix; cytoplasm
Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding
Biological Process: Wnt receptor signaling pathway; cell-cell signaling; regulation of cell growth; signal transduction; cell adhesion
Research Articles on WISP1
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Product Notes
The WISP1 wisp1 (Catalog #AAA176363) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-WISP1 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's WISP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC. Researchers should empirically determine the suitability of the WISP1 wisp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WISP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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