Mouse protein S (alpha) ELISA Kit | PROS1 elisa kit

Mouse Vitamin K-dependent protein S, PROS1 ELISA Kit

Cat. Num. AAA906634

• Gene Names: Pros1; AW214361
• Reactivity: Mouse
• Synonyms: protein S (alpha); Mouse Vitamin K-dependent protein S; PROS1 ELISA Kit; Mouse Vitamin K-dependent protein S (PROS1) ELISA kit; PROS; PS21; PS22; PS23; PS24; PS25; PSA; protein S; alpha; protein Sa; vitamin K-dependent plasma protein S; PROS1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse PROS1. No significant cross-reactivity or interference between mouse PROS1 and analogues was observed.
• Sequence Length: 675

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Competitive
• Detection Range: 6.25 ng/ml-400 ng/ml
• Sensitivity: < 1.56 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PROS1 elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PROS1. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for PROS1. The competitive inhibition reaction is launched between with pre-coated PROS1 and PROS1 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of PROS1 in the samples. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 46309455
• NCBI GeneID: 19128
• NCBI Accession #: NP_035303.1
• NCBI GenBank Nucleotide #: NM_011173.2
• UniProt Accession #: Q08761; P43483
• Molecular Weight: 74,934 Da
• NCBI Official Full Name: vitamin K-dependent protein S
• NCBI Official Synonym Full Names: protein S (alpha)
• NCBI Official Symbol: Pros1
• NCBI Official Synonym Symbols: AW214361
• NCBI Protein Information: vitamin K-dependent protein S
• UniProt Protein Name: Vitamin K-dependent protein S
• Protein Family: Proline--tRNA ligase
• UniProt Gene Name: Pros1
• UniProt Synonym Gene Names: Pros
• UniProt Entry Name: PROS_MOUSE

Uniprot Description

PROS1: Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis. Defects in PROS1 are the cause of thrombophilia due to protein S deficiency, autosomal dominant (THPH5). A hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. However, many adults with heterozygous disease may be asymptomatic. Based on the plasma levels of total and free PROS1 antigen as well as the serine protease-activated protein C cofactor activity, three types of PROS1D have been described: type I, characterized by reduced total and free PROS1 antigen levels together with reduced anticoagulant activity; type III, in which only free PROS1 antigen and PROS1 activity levels are reduced; and the rare type II which is characterized by normal concentrations of both total and free PROS1 antigen, but low cofactor activity. Defects in PROS1 are the cause of thrombophilia due to protein S deficiency, autosomal recessive (THPH6). A very rare and severe hematologic disorder resulting in thrombosis and secondary hemorrhage usually beginning in early infancy. Some affected individuals develop neonatal purpura fulminans, multifocal thrombosis, or intracranial hemorrhage.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; protein complex; extracellular region

Molecular Function: protein complex binding; calcium ion binding

Biological Process: fibrinolysis; hemostasis; positive regulation of phagocytosis; negative regulation of coagulation; blood coagulation