Human Vitamin K-dependent protein S ELISA Kit | PROS1 elisa kit

Human Vitamin K-dependent protein S ELISA Kit

Cat. Num. AAA9427967

• Gene Names: PROS1; PSA; PROS; PS21; PS22; PS23; PS24; PS25; THPH5; THPH6
• Reactivity: Human
• Synonyms: Vitamin K-dependent protein S; Human Vitamin K-dependent protein S ELISA Kit; PROS1; PROS; PROS1 elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human TNF alpha-induced protein 3. No significant cross-reactivity or interference was observed.

• Samples: Serum, Plasma, Tissue Homogenates And Cell Culture Supernates And Other Biological Fluids
• Detection Range: 6.25-400 ng/mL
• Sensitivity: <1.56 ng/mL
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PROS1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human tumor necrosis factor alpha-induced protein 3,TNF alpha-induced protein 3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNF alpha-induced protein 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TNF alpha-induced protein 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TNF alpha-induced protein 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TNF alpha-induced protein 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 192447438
• NCBI GeneID: 5627
• NCBI Accession #: NP_000304.2
• NCBI GenBank Nucleotide #: NM_000313.3
• UniProt Accession #: P07225; Q15518; Q7Z715; Q9UCZ8; A8KAC9; D3DN28
• Molecular Weight: 75,123 Da
• NCBI Official Full Name: vitamin K-dependent protein S isoform 2 preproprotein
• NCBI Official Synonym Full Names: protein S (alpha)
• NCBI Official Symbol: PROS1
• NCBI Official Synonym Symbols: PSA; PROS; PS21; PS22; PS23; PS24; PS25; THPH5; THPH6
• NCBI Protein Information: vitamin K-dependent protein S
• UniProt Protein Name: Vitamin K-dependent protein S
• Protein Family: Vitamin K-dependent protein
• UniProt Gene Name: PROS1
• UniProt Synonym Gene Names: PROS

NCBI Description

This gene encodes a vitamin K-dependent plasma protein that functions as a cofactor for the anticoagulant protease, activated protein C (APC) to inhibit blood coagulation. It is found in plasma in both a free, functionally active form and also in an inactive form complexed with C4b-binding protein. Mutations in this gene result in autosomal dominant hereditary thrombophilia. An inactive pseudogene of this locus is located at an adjacent region on chromosome 3. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Oct 2015]

Uniprot Description

PROS1: Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis. Defects in PROS1 are the cause of thrombophilia due to protein S deficiency, autosomal dominant (THPH5). A hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. However, many adults with heterozygous disease may be asymptomatic. Based on the plasma levels of total and free PROS1 antigen as well as the serine protease-activated protein C cofactor activity, three types of PROS1D have been described: type I, characterized by reduced total and free PROS1 antigen levels together with reduced anticoagulant activity; type III, in which only free PROS1 antigen and PROS1 activity levels are reduced; and the rare type II which is characterized by normal concentrations of both total and free PROS1 antigen, but low cofactor activity. Defects in PROS1 are the cause of thrombophilia due to protein S deficiency, autosomal recessive (THPH6). A very rare and severe hematologic disorder resulting in thrombosis and secondary hemorrhage usually beginning in early infancy. Some affected individuals develop neonatal purpura fulminans, multifocal thrombosis, or intracranial hemorrhage.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q11.1

Cellular Component: endoplasmic reticulum membrane; extracellular region; extracellular space; Golgi lumen; Golgi membrane; plasma membrane

Molecular Function: endopeptidase inhibitor activity

Biological Process: blood coagulation; ER to Golgi vesicle-mediated transport; leukocyte migration; peptidyl-glutamic acid carboxylation; platelet degranulation; regulation of complement activation; signal peptide processing

Disease: Thrombophilia Due To Protein S Deficiency, Autosomal Dominant; Thrombophilia Due To Protein S Deficiency, Autosomal Recessive