Peroxiredoxin 2 Recombinant Protein | PRDX2 recombinant protein

Peroxiredoxin 2 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123224

• Gene Names: Prdx2; TR; PRP; TPx; TSA; TDX1; NkefB; PrxII; TPx-B; Tdpx1; Torin; Band-8; AL022839
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Peroxiredoxin 2; Peroxiredoxin 2 Protein; Mouse; Recombinant (His Tag); Mouse Peroxiredoxin 2/PRDX2 Protein (His Tag); AL022839 Protein; Band-8 Protein; NkefB Protein; PRP Protein; PrxII Protein; Tdpx1 Protein; TDX1 Protein; Torin Protein; TPx Protein; TPx-B Protein; TR Protein; TSA Protein; peroxiredoxin 2; PRDX2 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, pH7.5,150mM NaCl,10% glycerol,0.5mM TCEP. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn198

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mouse PRDX2 (NP_035693.3)(Met1-Asn198) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PRDX2 recombinant protein

Background: Peroxiredoxin-2, also known as Natural killer cell-enhancing factor B, NKEF-B, Thiol-specific antioxidant protein, Thioredoxin peroxidase 1, Thioredoxin-dependent peroxide reductase 1, PRDX2 and NKEFB, is a cytoplasm protein that belongs to the ahpC/TSA family. Peroxiredoxin-2/PRDX2 contains one thioredoxin domain. Peroxiredoxin-2/PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. Peroxiredoxin-2/PRDX2 is not able to receive electrons from glutaredoxin. It may play an important role in eliminating peroxides generated during metabolism. Peroxiredoxin-2/PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. The Peroxiredoxins/Prx are a family of peroxidases that can reduce H2O2 using an electron from thioredoxin (Trx) or other substances. The mammalian Peroxiredoxins/Prx family is divided into six groups (PRDX1, PRDX2, PRDX3, PRDX4, PRDX5, PRDX6) on the basis of homology of amino acid sequences. They are located in the cytosol and play a role in the cell signaling system. All six mammalian peroxiredoxins are expressed in the lung. Peroxiredoxins/Prx is overexpressed in breast cancer tissues to a great extent suggesting that Peroxiredoxins/Prx has a proliferative effect and may be related to cancer development or progression.

References

Cha M.-K., et al., 1995, Biochem. Biophys. Res. Commun. 217:900-7. Noh,D.Y. et al., 2001, Anticancer Res. 21 (3B): 2085-90. Chevallet M., et al., 2003, J. Biol. Chem. 278:37146-53. Schremmer,B. et al., 2007, Subcell Biochem. 44 :317-44. Gauci S., et al., 2009, Anal. Chem. 81:4493-501.

NCBI and Uniprot Product Information

• NCBI GI #: 148747558
• NCBI GeneID: 21672
• NCBI Accession #: NP_035693.3
• NCBI GenBank Nucleotide #: NM_011563.6
• UniProt Accession #: Q61171; O88376; Q60796; Q9CWJ4; Q9DB49
• Molecular Weight: 21,779 Da
• NCBI Official Full Name: peroxiredoxin-2
• NCBI Official Synonym Full Names: peroxiredoxin 2
• NCBI Official Symbol: Prdx2
• NCBI Official Synonym Symbols: TR; PRP; TPx; TSA; TDX1; NkefB; PrxII; TPx-B; Tdpx1; Torin; Band-8; AL022839
• NCBI Protein Information: peroxiredoxin-2
• UniProt Protein Name: Peroxiredoxin-2
• Protein Family: Peroxiredoxin
• UniProt Gene Name: Prdx2
• UniProt Synonym Gene Names: Tdpx1; Tpx; TSA

Uniprot Description

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2.