Peroxiredoxin 2 Active Protein | PRDX2 active protein

Peroxiredoxin 2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120761

• Gene Names: PRDX2; PRP; TSA; PRX2; PTX1; TPX1; NKEFB; PRXII; TDPX1; NKEF-B
• Purity: >92% as determined by SDS-PAGE
• Synonyms: Peroxiredoxin 2; Peroxiredoxin 2 Protein; Human; Recombinant (His Tag); Human Peroxiredoxin 2/PRDX2 Protein (His Tag); HEL-S-2a Protein; NKEF-B Protein; NKEFB Protein; PRP Protein; PRX2 Protein; PRXII Protein; PTX1 Protein; TDPX1 Protein; TPX1 Protein; TSA Protein; peroxiredoxin 2; PRDX2 active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH8.0, 10% gly. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn198

• Species: Human
• Activity: Measured by its ability to reduce H2O2. The specific activity is >300pmoles/min/ug.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human PRDX2 (P32119)(Met 1-Asn 198) was expressed, with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PRDX2 active protein

Background: Peroxiredoxin-2, also known as Natural killer cell-enhancing factor B, NKEF-B, Thiol-specific antioxidant protein, Thioredoxin peroxidase 1, Thioredoxin-dependent peroxide reductase 1, PRDX2 and NKEFB, is a cytoplasm protein that belongs to the ahpC/TSA family. Peroxiredoxin-2/PRDX2 contains one thioredoxin domain. Peroxiredoxin-2/PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. Peroxiredoxin-2/PRDX2 is not able to receive electrons from glutaredoxin. It may play an important role in eliminating peroxides generated during metabolism. Peroxiredoxin-2/PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. The Peroxiredoxins/Prx are a family of peroxidases that can reduce H2O2 using an electron from thioredoxin (Trx) or other substances. The mammalian Peroxiredoxins/Prx family is divided into six groups (PRDX1, PRDX2, PRDX3, PRDX4, PRDX5, PRDX6) on the basis of homology of amino acid sequences. They are located in the cytosol and play a role in the cell signaling system. All six mammalian peroxiredoxins are expressed in the lung. Peroxiredoxins/Prx is overexpressed in breast cancer tissues to a great extent suggesting that Peroxiredoxins/Prx has a proliferative effect and may be related to cancer development or progression.

References

Cha M.-K., et al., 1995, Biochem. Biophys. Res. Commun. 217:900-7. Noh,D.Y. et al., 2001, Anticancer Res. 21 (3B): 2085-90. Chevallet M., et al., 2003, J. Biol. Chem. 278:37146-53. Schremmer,B. et al., 2007, Subcell Biochem. 44 :317-44. Gauci S., et al., 2009, Anal. Chem. 81:4493-501.

NCBI and Uniprot Product Information

• NCBI GI #: 49456535
• NCBI GeneID: 7001
• UniProt Accession #: P32119; P31945; P32118; P35701; Q6FHG4; Q92763; Q9UC23; A8K0C0
• Molecular Weight: 21,892 Da
• NCBI Official Full Name: PRDX2
• NCBI Official Synonym Full Names: peroxiredoxin 2
• NCBI Official Symbol: PRDX2
• NCBI Official Synonym Symbols: PRP; TSA; PRX2; PTX1; TPX1; NKEFB; PRXII; TDPX1; NKEF-B
• NCBI Protein Information: peroxiredoxin-2; torin; thioredoxin peroxidase 1; thiol-specific antioxidant 1; natural killer cell-enhancing factor B; thioredoxin-dependent peroxide reductase 1
• UniProt Protein Name: Peroxiredoxin-2
• Protein Family: Peroxiredoxin
• UniProt Gene Name: PRDX2
• UniProt Synonym Gene Names: NKEFB; TDPX1; NKEF-B; TSA
• UniProt Entry Name: PRDX2_HUMAN

NCBI Description

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein plays an antioxidant protective role in cells, and it may contribute to the antiviral activity of CD8(+) T-cells. The crystal structure of this protein has been resolved to 2.7 angstroms. This protein prevents hemolytic anemia from oxidative stress by stabilizing hemoglobin, thus making this gene a therapeutic target for patients with hemolytic anemia. This protein may have a proliferative effect and play a role in cancer development or progression. Related pseudogenes have been identified on chromosomes 5, 6, 10 and 13. [provided by RefSeq, Mar 2013]

Uniprot Description

PRDX2: a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). May have a proliferative effect and play a role in cancer development or progression. Three splice-variant isoforms have been identified.

Protein type: EC 1.11.1.15; Oxidoreductase

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cytoplasm; cytosol

Molecular Function: antioxidant activity; thioredoxin peroxidase activity

Biological Process: regulation of apoptosis; response to reactive oxygen species; removal of superoxide radicals; hydrogen peroxide catabolic process; negative regulation of neuron apoptosis; response to oxidative stress; negative regulation of apoptosis