Typical Testing Data/Standard Curve (for reference only)
Human HAMA ELISA Kit | HAMA elisa kit
HAMA ELISA
Reactivity
Human
Synonyms
HAMA; HAMA ELISA; HAMA elisa kit
Reactivity
Human
Intended Uses
This assay is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with an antibody, directed towards an epitope of an antigen molecule. An aliquot of unknown serum is incubated in the coated well with enzyme conjugated second antibody (E-Ab), directed towards a different region of the antigen molecule. After incubation the unbound E-Ab is washed off. The amount of bound E-Ab is proportional to the concentration of antigen in the sample. After adding the substrate solution, the intensity of colour developed is proportional to the antigen concentration in the sample. The measured ODs of the standards are used to construct a calibration curve against which the unknown samples are calculated.
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HAMA elisa kit
Background: The HAMA Enzyme Immunoassay Kit provides materials for the determination of human anti-mouse antibodies (HAMA) in serum. This assay is intended for research use only. HAMA are antibodies found in human serum which have the ability to bind to mouse immunoglobulin G (IgG). The presence of HAMA is the result of an immune reaction following an exposure to mice or other similar agent which was able to induce the immune system to generate an antibody able to bind to mouse IgG. HAMA is commonly found in patients following injection of murine monoclonal antibodies associated with some medical procedures. Some human auto-antibodies, the most common of which is rheumatoid factor (Rf), by virtue of their cross reactivity are able to bind mouse IgG. The HAMA assay has been designed to not be affected by rheumatoid factor. The HAMA assay measures HAMA across a wide dynamic range and it is not necessary to predilute human serum or plasma samples before the HAMA assay. However, if an elevated HAMA sample is diluted with the HAMA zero calibrator the HAMA assay has been demonstrated to yield a linear dilution of HAMA.
Product Categories/Family for HAMA elisa kit
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Product Notes
The Human HAMA (Catalog #AAA494666-IDC-14) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494666-IDC-14 ELISA Kit recognizes Human HAMA. It is sometimes possible for the material contained within the vial of "HAMA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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