Peroxiredoxin 1 (PRDX1) Recombinant Protein | PRDX1 recombinant protein

Recombinant Peroxiredoxin 1 (PRDX1)

Cat. Num. AAA2011484

• Gene Names: Prdx1; PAG; OSF3; Paga; PrxI; TDX2; TPxA; prx1; MSP23; NkefA; OSF-3; PrdxI; Tdpx2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Peroxiredoxin 1 (PRDX1); Recombinant Peroxiredoxin 1 (PRDX1); PRDX1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- MSSGNAKIGY PAPNFKATAV MPDGQFKDIS LSEYKGKYVV FFFYPLDFTF VCPTEIIAFSDRADEFKKLN CQVIGASVDS HFCHLAWINT PKKQGGLGPM NIPLISDPKR TIAQDYGVLK ADEGISFRGL FIIDDKGILR QITINDLPVG RSVDEIIRLV QAFQFTDKHG EVCPAGWKPG SDTIKPDVNK SKEYFSKQK
• Sequence Length: 199

• Applicable Applications for PRDX1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Met1-Lys199 (Accession # P35700) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6754976
• NCBI GeneID: 18477
• NCBI Accession #: NP_035164.1
• NCBI GenBank Nucleotide #: NM_011034.4
• UniProt Accession #: P35700; Q3UBV4; Q9CWI2
• Molecular Weight: 49.2kDa
• NCBI Official Full Name: peroxiredoxin-1
• NCBI Official Synonym Full Names: peroxiredoxin 1
• NCBI Official Symbol: Prdx1
• NCBI Official Synonym Symbols: PAG; OSF3; Paga; PrxI; TDX2; TPxA; prx1; MSP23; NkefA; OSF-3; PrdxI; Tdpx2
• NCBI Protein Information: peroxiredoxin-1; thioredoxin peroxidase 2; osteoblast specific factor 3; osteoblast-specific factor 3; macrophage 23 Kd stress protein; macrophage 23kDa stress protein; macrophase stress protein 22kDa; macrophase stress protein 23 kd; proliferation-associated gene A; macrophage 23 kDa stress protein; Trx dependent peroxide reductase 2; thioredoxin dependent peroxide reductase 2; thioredoxin-dependent peroxide reductase 2
• UniProt Protein Name: Peroxiredoxin-1
• Protein Family: Peroxiredoxin
• UniProt Gene Name: Prdx1
• UniProt Synonym Gene Names: Msp23; Paga; Tdpx2; OSF-3
• UniProt Entry Name: PRDX1_MOUSE

Uniprot Description

PRDX1: a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). May have a proliferative effect and play a role in cancer development or progression.

Protein type: Nuclear receptor co-regulator; EC 1.11.1.15; Oxidoreductase

Cellular Component: extracellular space; peroxisomal matrix; mitochondrion; mitochondrial matrix; cytoplasm; nucleolus; nucleus; cytosol; myelin sheath

Molecular Function: antioxidant activity; identical protein binding; protein binding; peroxidase activity; protein homodimerization activity; thioredoxin peroxidase activity; peroxiredoxin activity; heme binding; oxidoreductase activity

Biological Process: regulation of stress-activated MAPK cascade; cell proliferation; erythrocyte homeostasis; response to reactive oxygen species; removal of superoxide radicals; natural killer cell mediated cytotoxicity; hydrogen peroxide catabolic process; response to oxidative stress; regulation of NF-kappaB import into nucleus