PRDX1 active protein

PRDX1, 1-199aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA203281

• Gene Names: PRDX1; PAG; PAGA; PAGB; PRX1; PRXI; MSP23; NKEFA; TDPX2; NKEF-A
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: PRDX1; 1-199aa; Human; His tag; E Coli (Bioactivity Validated); Peroxiredoxin 1; MSP23; NKEFA; PAG; PAGA; PAGB; PRX1; PRXI; TDPX2; Thioredoxin peroxidase 2; Peroxiredoxin 1 Hbp23; Heme binding 23 kDa protein; Paga; Prdx 1; Macrophage 23 Kd stress protein; NKEF A; NkefA; OSF3; Macrophage 23kDa stress protein; PrxI; Tdpx 2; TDX2; Macrophase stress protein 22kDa; TPxA Macrophase stress protein 23 kd; Natural killer cell enhancing factor A; Natural killer enhancing factor A; Osteoblast specific factor 3; Proliferation associated gene A; Proliferation associated protein PAG; Thioredoxin dependent peroxide reductase 2; ; PRDX1 active protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid in 20 mM Tris, pH 7.5, 20% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSSGNAKIGH PAPNFKATAV MPDGQFKDIS LSDYKGKYVV FFFYPLDFTF VCPTEIIAFS DRAEEFKKLN CQVIGASVDS HFCHLAWVNT PKKQGGLGPM NIPLVSDPKR TIAQDYGVLK ADEGISFRGL FIIDDKGILR QITVNDLPVG RSVDETLRLV QAFQFTDKHG EVCPAGWKPG SDTIKPDVQK SKEYFSKQK
• Sequence Length: 199

• Applicable Applications for PRDX1 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity is >2,000 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for minute.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PRDX1 active protein

PRDX1 is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. This protein is also known to be important to protect red blood cells against reactive oxygen species and in tumor prevention. Recombinant human PRDX1, fused to His-tag at N-terminus, was expressed in E Coli and purified by conventional chromatography techniques.

Product Categories/Family for PRDX1 active protein

Redox Proteins

References

Iraqui I., et al. (2008) Cancer Res. 68 (4):1055-63.; Parmigiani RB., et al. (2008) Proc Natl Acad Sci u S A. 105 (28):9633-8.; Kim JH., et al. (2008) Clin Cancer Res. 14 (8):2326-33.;

NCBI and Uniprot Product Information

• NCBI GI #: 4505591
• NCBI GeneID: 5052
• NCBI Accession #: NP_002565
• NCBI GenBank Nucleotide #: NM_002574.3
• UniProt Accession #: Q06830; P35703; Q2V576; Q5T154; Q5T155; B5BU26; D3DPZ8
• Molecular Weight: 24 kDa (219 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: peroxiredoxin-1
• NCBI Official Synonym Full Names: peroxiredoxin 1
• NCBI Official Symbol: PRDX1
• NCBI Official Synonym Symbols: PAG; PAGA; PAGB; PRX1; PRXI; MSP23; NKEFA; TDPX2; NKEF-A
• NCBI Protein Information: peroxiredoxin-1
• UniProt Protein Name: Peroxiredoxin-1
• Protein Family: Peroxiredoxin
• UniProt Gene Name: PRDX1
• UniProt Synonym Gene Names: PAGA; PAGB; TDPX2; NKEF-A; PAG

NCBI Description

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2 (PubMed:9497357). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation ().