Mouse peroxiredoxin 1 ELISA Kit | PRDX1 elisa kit

Mouse Peroxiredoxin-1, PRDX1 ELISA Kit

Cat. Num. AAA907485

• Gene Names: Prdx1; PAG; OSF3; Paga; PrxI; TDX2; TPxA; prx1; MSP23; NkefA; OSF-3; PrdxI; Tdpx2
• Reactivity: Mouse
• Synonyms: peroxiredoxin 1; Mouse Peroxiredoxin-1; PRDX1 ELISA Kit; Mouse Peroxiredoxin-1 (PRDX1) ELISA kit; MSP23; NKEFA; PAG; PAGA; PAGB; PRX1; PRXI; TDPX2; natural killer-enhancing factor A; proliferation-associated gene A; thioredoxin-dependent peroxide reductase 2; PRDX1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse PRDX1. No significant cross-reactivity or interference between mouse PRDX1 and analogues was observed.
• Sequence Length: 199

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 0.312 ng/ml -20 ng/ml.
• Sensitivity: The minimum detectable dose of mouse PRDX1 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRDX1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRDX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRDX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDX1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6754976
• NCBI GeneID: 18477
• NCBI Accession #: NP_035164.1
• NCBI GenBank Nucleotide #: NM_011034.4
• UniProt Accession #: P35700; Q3UBV4; Q9CWI2
• Molecular Weight: 22,176 Da
• NCBI Official Full Name: peroxiredoxin-1
• NCBI Official Synonym Full Names: peroxiredoxin 1
• NCBI Official Symbol: Prdx1
• NCBI Official Synonym Symbols: PAG; OSF3; Paga; PrxI; TDX2; TPxA; prx1; MSP23; NkefA; OSF-3; PrdxI; Tdpx2
• NCBI Protein Information: peroxiredoxin-1; thioredoxin peroxidase 2; osteoblast specific factor 3; osteoblast-specific factor 3; macrophage 23 Kd stress protein; macrophage 23kDa stress protein; macrophase stress protein 22kDa; macrophase stress protein 23 kd; proliferation-associated gene A; macrophage 23 kDa stress protein; Trx dependent peroxide reductase 2; thioredoxin dependent peroxide reductase 2; thioredoxin-dependent peroxide reductase 2
• UniProt Protein Name: Peroxiredoxin-1
• Protein Family: Peroxiredoxin
• UniProt Gene Name: Prdx1
• UniProt Synonym Gene Names: Msp23; Paga; Tdpx2; OSF-3
• UniProt Entry Name: PRDX1_MOUSE

Uniprot Description

PRDX1: a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). May have a proliferative effect and play a role in cancer development or progression.

Protein type: Oxidoreductase; Nuclear receptor co-regulator; EC 1.11.1.15

Cellular Component: peroxisomal matrix; extracellular space; mitochondrion; mitochondrial matrix; cytoplasm; nucleolus; cytosol; nucleus; myelin sheath

Molecular Function: antioxidant activity; identical protein binding; protein binding; peroxidase activity; protein homodimerization activity; peroxiredoxin activity; thioredoxin peroxidase activity; heme binding; oxidoreductase activity

Biological Process: regulation of stress-activated MAPK cascade; erythrocyte homeostasis; cell proliferation; response to reactive oxygen species; removal of superoxide radicals; natural killer cell mediated cytotoxicity; hydrogen peroxide catabolic process; response to oxidative stress; regulation of NF-kappaB import into nucleus