Typical Testing Data/Standard Curve (for reference only)
Mouse prolylcarboxypeptidase (angiotensinase C) ELISA Kit | PRCP elisa kit
Mouse Lysosomal Pro-X carboxypeptidase, PRCP ELISA Kit
Gene Names
Prcp; PCP; HUMPCP; AI451719; 2510048K03Rik; 2610104A14Rik
Reactivity
Mouse
Synonyms
prolylcarboxypeptidase (angiotensinase C); Mouse Lysosomal Pro-X carboxypeptidase; PRCP ELISA Kit; Mouse Lysosomal Pro-X carboxypeptidase (PRCP) ELISA kit; HUMPCP; MGC2202; PCP; angiotensinase C; lysosomal Pro-x carboxypeptidase; lysosomal carboxypeptidase C; proline carboxypeptidase; prolylcarboxypeptidase; prolylcarboxypeptidase isoform 1 preproprotein; PRCP elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse PRCP. No significant cross-reactivity or interference between mouse PRCP and analogues was observed.
Sequence Length
491
Samples
Serum, plasma, tissue homogenates and cell lysates
Assay Type
Quantitative Sandwich
Detection Range
37.5 pg/ml-2400 pg/ml
Sensitivity
< 9.37 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PRCP elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRCP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRCP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRCP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRCP bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,027 Da
NCBI Official Full Name
lysosomal Pro-X carboxypeptidase
NCBI Official Synonym Full Names
prolylcarboxypeptidase (angiotensinase C)
NCBI Official Symbol
Prcp
NCBI Official Synonym Symbols
PCP; HUMPCP; AI451719; 2510048K03Rik; 2610104A14Rik
NCBI Protein Information
lysosomal Pro-X carboxypeptidase; proline carboxypeptidase
UniProt Protein Name
Lysosomal Pro-X carboxypeptidase
Protein Family
UniProt Gene Name
Prcp
UniProt Synonym Gene Names
PRCP
UniProt Entry Name
PCP_MOUSE
Uniprot Description
PRCP: Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH. Belongs to the peptidase S28 family.
Protein type: Protease; EC 3.4.16.2
Cellular Component: lysosome
Molecular Function: peptidase activity; carboxypeptidase activity; hydrolase activity; serine-type peptidase activity
Biological Process: plasma kallikrein-kinin cascade; negative regulation of systemic arterial blood pressure; proteolysis; angiogenesis involved in wound healing; regulation of blood vessel endothelial cell migration; glucose homeostasis
Research Articles on PRCP
Similar Products
Product Notes
The Mouse PRCP prcp (Catalog #AAA929190) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA929190 ELISA Kit recognizes Mouse PRCP. It is sometimes possible for the material contained within the vial of "prolylcarboxypeptidase (angiotensinase C), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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