Western Blot (WB)
(Western Blot: Sample: Mouse Kidney lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse PRCP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Prolylcarboxypeptidase Polyclonal Antibody | anti-PRCP antibody
Polyclonal Antibody to Prolylcarboxypeptidase (PRCP)
Gene Names
Prcp; PCP; HUMPCP; AI451719; 2510048K03Rik; 2610104A14Rik
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Prolylcarboxypeptidase; Polyclonal Antibody; Polyclonal Antibody to Prolylcarboxypeptidase (PRCP); anti-PRCP antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-PRCP antibody
WB; IHC; ICC; IP.
Application Notes
Western blotting: 0.5-2 Ug/mL;1:500-2000
Immunohistochemistry: 5-20 Ug/mL;1:50-200
Immunocytochemistry: 5-20 Ug/mL;1:50-200
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20 Ug/mL;1:50-200
Immunocytochemistry: 5-20 Ug/mL;1:50-200
Optimal working dilutions must be determined by end user.
Organism Species
Mus musculus (Mouse)
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant PRCP (Gln169~Leu443) expressed in E.coli
Cross Reactivity
Mouse
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Mouse Kidney lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse PRCP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Kidney lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse PRCP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant PRCP, Mouse))
Western Blot (WB)
(Western Blot: Sample: Recombinant PRCP, Mouse))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
lysosomal Pro-X carboxypeptidase
NCBI Official Synonym Full Names
prolylcarboxypeptidase (angiotensinase C)
NCBI Official Symbol
Prcp
NCBI Official Synonym Symbols
PCP; HUMPCP; AI451719; 2510048K03Rik; 2610104A14Rik
NCBI Protein Information
lysosomal Pro-X carboxypeptidase
UniProt Protein Name
Lysosomal Pro-X carboxypeptidase
Protein Family
UniProt Gene Name
Prcp
UniProt Synonym Gene Names
PRCP
Uniprot Description
PRCP: Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH. Belongs to the peptidase S28 family.
Protein type: EC 3.4.16.2; Protease
Chromosomal Location of Human Ortholog: 7|7 E1
Cellular Component: lysosome
Molecular Function: carboxypeptidase activity; dipeptidyl-peptidase activity; hydrolase activity; peptidase activity; serine-type peptidase activity
Biological Process: angiogenesis involved in wound healing; glucose homeostasis; negative regulation of systemic arterial blood pressure; plasma kallikrein-kinin cascade; proteolysis; regulation of blood vessel endothelial cell migration
Research Articles on PRCP
Similar Products
Product Notes
The PRCP prcp (Catalog #AAA2026156) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Prolylcarboxypeptidase (PRCP) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Prolylcarboxypeptidase can be used in a range of immunoassay formats including, but not limited to, WB; IHC; ICC; IP. Western blotting: 0.5-2 Ug/mL;1:500-2000 Immunohistochemistry: 5-20 Ug/mL;1:50-200 Immunocytochemistry: 5-20 Ug/mL;1:50-200 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the PRCP prcp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prolylcarboxypeptidase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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