Human Prolylcarboxypeptidase ELISA Kit | PRCP elisa kit

Human Prolylcarboxypeptidase ELISA Kit

Cat. Num. AAA762977

• Gene Names: PRCP; PCP; HUMPCP
• Reactivity: Human
• Synonyms: Prolylcarboxypeptidase; Human Prolylcarboxypeptidase ELISA Kit; PRCP/Angiotensinase C/HUMPCP/Angiotensinase C/HUMPCP/Lysosomal carboxypeptidase C/lysosomal Pro-X carboxypeptidase/Proline carboxypeptidase/Prolylcarboxypeptidase/prolylcarboxypeptidase (angiotensinase C)/prolylcarboxypeptidase isoform 1 preproprotein; PRCP elisa kit

• Reactivity: Human
• Sequence Length: 496

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PRCP elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human PRCP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PRCP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PRCP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PRCP can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 431321
• NCBI GeneID: 5547
• Molecular Weight: 58,100 Da
• NCBI Official Full Name: prolylcarboxypeptidase
• NCBI Official Synonym Full Names: prolylcarboxypeptidase
• NCBI Official Symbol: PRCP
• NCBI Official Synonym Symbols: PCP; HUMPCP
• NCBI Protein Information: lysosomal Pro-X carboxypeptidase
• UniProt Protein Name: Lysosomal Pro-X carboxypeptidase
• Protein Family: Lysosomal Pro-X carboxypeptidase
• UniProt Gene Name: PRCP
• UniProt Synonym Gene Names: PCP; PRCP
• UniProt Entry Name: PCP_HUMAN

NCBI Description

This gene encodes a member of the peptidase S28 family of serine exopeptidases. The encoded preproprotein is proteolytically processed to generate the mature lysosomal prolylcarboxypeptidase. This enzyme cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. A pseudogene of this gene has been identified on chromosome 2. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

Uniprot Description

PRCP: Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH. Belongs to the peptidase S28 family.

Protein type: EC 3.4.16.2; Protease

Chromosomal Location of Human Ortholog: 11q14

Cellular Component: lysosome; plasma membrane

Molecular Function: dipeptidyl-peptidase activity; protein binding; serine carboxypeptidase activity

Biological Process: angiogenesis involved in wound healing; blood coagulation; blood coagulation, intrinsic pathway; glucose homeostasis; negative regulation of systemic arterial blood pressure; plasma kallikrein-kinin cascade; proteolysis; regulation of blood vessel endothelial cell migration