Western Blot (WB)
(Western blot analysis of extracts from COLO cells, using RAB6C antibody.)
Rabbit RAB6C Polyclonal Antibody | anti-RAB6C antibody
RAB6C Antibody
Gene Names
RAB6C; WTH3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
RAB6C; Polyclonal Antibody; RAB6C Antibody; RAB 6C; Rab6 like protein WTH3; Rab6-like protein WTH3; RAB6C member RAS oncogene family; RAB6C_HUMAN; Ras related protein Rab 6C; Ras-related protein Rab-6C; WTH3; anti-RAB6C antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
RAB6C antibody detects endogenous levels of total RAB6C
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
254
Applicable Applications for anti-RAB6C antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cytoskeleton; Nucleus
Tissue Specificity
Highest levels are found in fetal and adult brain, prostate, testis and spinal cord. Undetectable expression in adrenal gland, skeletal muscle, bone marrow, fetal, and adult liver, heart, salivary gland, and trachea. Detected in the HEK293, HEK293T, LNCaP, MCF-7, T-47D and EVSA-T cell lines (at protein level).
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from COLO cells, using RAB6C antibody.)
Western Blot (WB)
(Western blot analysis of extracts from COLO cells, using RAB6C antibody.)
Immunofluorescene (IF)
(MBS9605862 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescene (IF)
(MBS9605862 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-RAB6C antibody
Function: May be involved in the regulation of centrosome duplication and cell cycle progression.
Similarity: Belongs to the small GTPase superfamily. Rab family.
Similarity: Belongs to the small GTPase superfamily. Rab family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 28 kD
Predicted: 29 kDa
Predicted: 29 kDa
NCBI Official Full Name
ras-related protein Rab-6C
NCBI Official Synonym Full Names
RAB6C, member RAS oncogene family
NCBI Official Symbol
RAB6C
NCBI Official Synonym Symbols
WTH3
NCBI Protein Information
ras-related protein Rab-6C
UniProt Protein Name
Ras-related protein Rab-6C
Protein Family
UniProt Gene Name
RAB6C
UniProt Synonym Gene Names
WTH3
Uniprot Description
May be involved in the regulation of centrosome duplication and cell cycle progression.
Research Articles on RAB6C
Similar Products
Product Notes
The RAB6C rab6c (Catalog #AAA9605862) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAB6C Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RAB6C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the RAB6C rab6c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB6C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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