Mouse Heat Shock Protein 90kDa Alpha A1 (HSP90aA1) ELISA Kit | HSP90aA1 elisa kit

Mouse Heat Shock Protein 90kDa Alpha A1 (HSP90aA1) ELISA Kit

Cat. Num. AAA2022809

• Reactivity: Mouse
• Synonyms: Heat Shock Protein 90kDa Alpha A1 (HSP90aA1); Mouse Heat Shock Protein 90kDa Alpha A1 (HSP90aA1) ELISA Kit; HSP90aA1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of HSP90aA1. No significant cross-reactivity or interference between HSP90aA1 and analogues was observed.

• Samples: Mouse serum, plasma, tissue homogenates, Cell lysates and other biological fluids
• Detection Range: 78-5,000pg/mL
• Sensitivity: The minimum detectable dose of HSP90aA1 is typically less than 31pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HSP90aA1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HSP90aA1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HSP90aA1 in mouse serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HSP90aA1 elisa kit

Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSP90aA1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HSP90aA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HSP90aA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HSP90aA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.