Western Blot (WB)
(Western blot analysis of RHBDD1 expression in Raji (A), mouse kidney (B), rat brain (C) whole cell lysates.)
Rabbit RHBDD1 Polyclonal Antibody | anti-RHBDD1 antibody
Anti-RHBDD1 Antibody
IF/ICC: 1:50-1:200
Western Blot (WB)
(Western blot analysis of RHBDD1 expression in Raji (A), mouse kidney (B), rat brain (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of RHBDD1 expression in Raji (A), mouse kidney (B), rat brain (C) whole cell lysates.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Immunofluorescent analysis of RHBDD1 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Immunofluorescent analysis of RHBDD1 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
Uniprot Description
RHBDD1: Intramembrane-cleaving serine protease that cleaves single transmembrane or multi-pass membrane proteins in the hydrophobic plane of the membrane, luminal loops and juxtamembrane regions. Involved in regulated intramembrane proteolysis and the subsequent release of functional polypeptides from their membrane anchors. Functional component of endoplasmic reticulum-associated degradation (ERAD) for misfolded membrane proteins. Required for the degradation process of some specific misfolded endoplasmic reticulum (ER) luminal proteins. Participates in the transfer of misfolded proteins from the ER to the cytosol, where they are destroyed by the proteasome in a ubiquitin-dependent manner. Functions in BIK, MPZ, PKD1, PTCRA, RHO, STEAP3 and TRAC processing. Involved in the regulation of exosomal secretion; inhibits the TSAP6-mediated secretion pathway. Involved in the regulation of apoptosis; modulates BIK-mediated apoptotic activity. Also plays a role in the regulation of spermatogenesis; inhibits apoptotic activity in spermatogonia. Belongs to the peptidase S54 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.21.105; Membrane protein, integral; Membrane protein, multi-pass; Protease
Chromosomal Location of Human Ortholog: 2q36.3
Cellular Component: endoplasmic reticulum membrane; mitochondrion; integral to endoplasmic reticulum membrane
Molecular Function: protein binding; serine-type endopeptidase activity; endopeptidase activity
Biological Process: spermatid differentiation; ER-associated protein catabolic process; membrane protein intracellular domain proteolysis; apoptosis; membrane protein proteolysis; positive regulation of protein catabolic process; positive regulation of secretion; post-translational protein modification; negative regulation of apoptosis
Research Articles on RHBDD1
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Product Notes
The RHBDD1 rhbdd1 (Catalog #AAA8307929) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RHBDD1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RHBDD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500-1:2000 IF/ICC: 1:50-1:200. Researchers should empirically determine the suitability of the RHBDD1 rhbdd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RHBDD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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