Chicken Phosphoenolpyruvate carboxykinase [GTP], mitochondrial ELISA Kit | PCK2 elisa kit

Chicken Phosphoenolpyruvate carboxykinase [GTP], mitochondrial ELISA Kit

Cat. Num. AAA288489

• Gene Names: PCK2; PEPCK-M
• Reactivity: Chicken
• Synonyms: Phosphoenolpyruvate carboxykinase [GTP]; mitochondrial; Chicken Phosphoenolpyruvate carboxykinase [GTP]; mitochondrial ELISA Kit; PCK2 elisa kit

• Reactivity: Chicken
• Specificity: This assay recognizes recombinant and natural Chicken PEPCK-M. No significant cross-reactivity or interference was observed.
• Sequence Length: 10

• Samples: Serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
• Assay Type: Competitive
• Detection Range: 0.78-50.0 ng/ml.
• Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of chicken PEPCK-M concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PCK2 elisa kit

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PEPCK-M, During the reaction, PEPCK-M in the sample or standard competes with a fixed amount of biotin-labeled PEPCK-M for sites on a pre-coated Monoclonal antibody specific to PEPCK-M. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PEPCK-M in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 45382653
• NCBI GeneID: 396457
• NCBI Accession #: NP_990801.1
• NCBI GenBank Nucleotide #: NM_205470.1
• UniProt Accession #: P21642
• Molecular Weight: 71,106 Da
• NCBI Official Full Name: phosphoenolpyruvate carboxykinase
• NCBI Official Synonym Full Names: phosphoenolpyruvate carboxykinase 2 (mitochondrial)
• NCBI Official Symbol: PCK2
• NCBI Official Synonym Symbols: PEPCK-M
• NCBI Protein Information: phosphoenolpyruvate carboxykinase [GTP], mitochondrial
• UniProt Protein Name: Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
• Protein Family: Protein kinase
• UniProt Gene Name: PCK2
• UniProt Synonym Gene Names: PEPCK-M
• UniProt Entry Name: PCKGM_CHICK

Uniprot Description

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Facilitates the recycling of lactate carbon in the liver.