Typical Testing Data/Standard Curve (for reference only)
Human Parathyroid hormone ELISA Kit | PTH elisa kit
Human Parathyroid hormone ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PTH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PTH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PTH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the parathyroid family of proteins. The encoded preproprotein is proteolytically processed to generate a protein that binds to the parathyroid hormone/parathyroid hormone-related peptide receptor and regulates blood calcium and phosphate levels. Excess production of the encoded protein, known as hyperparathyroidism, can result in hypercalcemia and kidney stones. On the other hand, defective processing of the encoded protein may lead to hypoparathyroidism, which can result in hypocalcemia and numbness. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
Uniprot Description
PTH: PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2- deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells. Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH); also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism. Belongs to the parathyroid hormone family.
Protein type: Hormone; Secreted; Secreted, signal peptide; Apoptosis
Chromosomal Location of Human Ortholog: 11p15.3-p15.1
Cellular Component: extracellular region; extracellular space; intracellular
Molecular Function: hormone activity; parathyroid hormone receptor binding; peptide hormone receptor binding; RNA polymerase II transcription factor activity, enhancer binding; type 1 parathyroid hormone receptor binding
Biological Process: bone resorption; cAMP metabolic process; cell-cell signaling; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; G-protein signaling, adenylate cyclase activating pathway; induction of apoptosis by hormones; negative regulation of transcription from RNA polymerase II promoter; positive regulation of bone mineralization; positive regulation of cAMP biosynthetic process; positive regulation of glucose import; positive regulation of glycogen biosynthetic process; positive regulation of signal transduction; positive regulation of transcription from RNA polymerase II promoter; regulation of gene expression; response to cadmium ion; response to drug; response to ethanol; response to lead ion; response to vitamin D; Rho protein signal transduction; skeletal development
Disease: Hypoparathyroidism, Familial Isolated
Research Articles on PTH
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Product Notes
The Human PTH pth (Catalog #AAA2880569) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2880569 ELISA Kit recognizes Human PTH. It is sometimes possible for the material contained within the vial of "Parathyroid hormone, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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