Rabbit anti-Human Zyxin Polyclonal Antibody | anti-Zyxin antibody

Anti-Zyxin Antibody

Cat. Num. AAA415590

• Gene Names: ZYX; ESP-2; HED-2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: Protein A purified
• Synonyms: Zyxin; Polyclonal Antibody; Anti-Zyxin Antibody; anti-Zyxin antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 572

• Applicable Applications for anti-Zyxin antibody: Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Recommended dilutions:; Peptide ELISA: 1:32,000; IF: 1:50; WB: 1:500 - 1:1000; Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Synthetic peptide conjugated to KLH
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Testing Data #2

Related Product Information for anti-Zyxin antibody

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.

References

Reinhard M, Jouvenal K, Tripier D Identification,purification,andcharacterization of a zyxin-related protein that binds the focal adhesion and microfilament protein VASP(vasodilator-stimulated phosphoprotein) Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7956-60

NCBI and Uniprot Product Information

• NCBI GI #: 58530845
• NCBI GeneID: 7791
• NCBI Accession #: NP_001010972.1
• NCBI GenBank Nucleotide #: NM_001010972.1
• Molecular Weight: 45,075 Da
• NCBI Official Full Name: zyxin
• NCBI Official Synonym Full Names: zyxin
• NCBI Official Symbol: ZYX
• NCBI Official Synonym Symbols: ESP-2; HED-2
• NCBI Protein Information: zyxin; zyxin-2
• UniProt Protein Name: Zyxin
• Protein Family: Zyxin
• UniProt Gene Name: ZYX
• UniProt Entry Name: ZYX_HUMAN

NCBI Description

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq, Jul 2008]

Uniprot Description

Zyxin: Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression. Interacts with HPV type 6 protein E6. Does not interact significantly with E6 proteins from HPV types 11, 16, or 18. Interacts, via the Pro-rich regions, with the EVH1 domains of ENAH, EVL and VASP. Interacts with the first LIM domain of TES. Interacts with NEBL (isoform 2). Belongs to the zyxin/ajuba family.

Protein type: Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: cell-cell adherens junction; focal adhesion; integral to plasma membrane; cytoplasm; plasma membrane; stress fiber; nucleus

Molecular Function: protein binding; zinc ion binding

Biological Process: integrin-mediated signaling pathway; cell-cell signaling; viral reproduction; transforming growth factor beta receptor signaling pathway; cell-matrix adhesion; regulation of inflammatory response; stress fiber formation; signal transduction; cell adhesion