Rabbit anti-Human, Mouse CA3 Polyclonal Antibody | anti-CA3 antibody

CA3, ID (CA3, Carbonic anhydrase 3, Carbonate dehydratase III, Carbonic anhydrase III) (HRP)

Cat. Num. AAA6280225

• Gene Names: CA3; Car3; CAIII
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: CA3; Polyclonal Antibody; ID (CA3; Carbonic anhydrase 3; Carbonate dehydratase III; Carbonic anhydrase III) (HRP); Carbonic anhydrase III; anti-CA3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CA3. Species Crossreactivity: mouse
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CA3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154~184 amino acids from the Central region of human CA3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of CA3 Antibody (Center) in mouse liver tissue lysates (35ug/lane). CA3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(CA3 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CA3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-CA3 antibody

Antibodies; Enzymes

References

Du, A.L., Autoimmunity 42 (3), 209-215 (2009). Dai, H.Y., Mol. Carcinog. 47 (12), 956-963 (2008). Gailly, P., Kidney Int. 74 (1), 52-61 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4885099
• NCBI GeneID: 761
• NCBI Accession #: NP_005172.1
• NCBI GenBank Nucleotide #: NM_005181.3
• UniProt Accession #: P07451; O60842; B2R867; B3KUC8
• Molecular Weight: 29,557 Da
• NCBI Official Full Name: carbonic anhydrase 3
• NCBI Official Synonym Full Names: carbonic anhydrase III, muscle specific
• NCBI Official Symbol: CA3
• NCBI Official Synonym Symbols: Car3; CAIII
• NCBI Protein Information: carbonic anhydrase 3; CA-III; carbonate dehydratase III
• UniProt Protein Name: Carbonic anhydrase 3
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA3
• UniProt Synonym Gene Names: CA-III
• UniProt Entry Name: CAH3_HUMAN

NCBI Description

Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008]

Uniprot Description

CA3: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.

Protein type: EC 4.2.1.1; Lyase; Energy Metabolism - nitrogen

Chromosomal Location of Human Ortholog: 8q21.2

Cellular Component: cytosol

Molecular Function: carbonate dehydratase activity; zinc ion binding; nickel ion binding

Biological Process: response to ethanol; bicarbonate transport; one-carbon compound metabolic process; response to oxidative stress