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SDS-Page (Gel: 6%SDS-PAGE Lysate: 40 ug, Lane: 293T cell lysate, Primary antibody:ZYX antibody at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds)

Rabbit anti-Human ZYX Polyclonal Antibody | anti-ZYX antibody

ZYX Antibody

Gene Names
ZYX; ESP-2; HED-2
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antigen affinity purification
Synonyms
ZYX; Polyclonal Antibody; ZYX Antibody; ESP-2; HED-2; anti-ZYX antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total ZYX protein.
Purity/Purification
Antigen affinity purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
0.7mg/ml (varies by lot)
Sequence Length
572
Applicable Applications for anti-ZYX antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
Western Blot: 1:200-1000
Immunohistochemistry: 1: 20-100
Immunogen
Fusion protein of human ZYX
Immunogen Type
Protein
Preparation and Storage
Store at -20 degree C

SDS-Page

(Gel: 6%SDS-PAGE Lysate: 40 ug, Lane: 293T cell lysate, Primary antibody:ZYX antibody at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds)

SDS-Page (Gel: 6%SDS-PAGE Lysate: 40 ug, Lane: 293T cell lysate, Primary antibody:ZYX antibody at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds)

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using ZYX Antibody at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200))

Immunohistochemistry (IHC) (The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using ZYX Antibody at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200))
Related Product Information for anti-ZYX antibody
Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.
Product Categories/Family for anti-ZYX antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
45,075 Da
NCBI Official Full Name
zyxin
NCBI Official Synonym Full Names
zyxin
NCBI Official Symbol
ZYX
NCBI Official Synonym Symbols
ESP-2; HED-2
NCBI Protein Information
zyxin
UniProt Protein Name
Zyxin
Protein Family
UniProt Gene Name
ZYX
UniProt Entry Name
ZYX_HUMAN

NCBI Description

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq, Jul 2008]

Uniprot Description

Zyxin: Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression. Interacts with HPV type 6 protein E6. Does not interact significantly with E6 proteins from HPV types 11, 16, or 18. Interacts, via the Pro-rich regions, with the EVH1 domains of ENAH, EVL and VASP. Interacts with the first LIM domain of TES. Interacts with NEBL (isoform 2). Belongs to the zyxin/ajuba family.

Protein type: Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: cell-cell adherens junction; cytoplasm; focal adhesion; integral to plasma membrane; nucleus; plasma membrane; stress fiber

Molecular Function: protein binding

Biological Process: cell adhesion; cell-cell signaling; cell-matrix adhesion; integrin-mediated signaling pathway; signal transduction; stress fiber formation; transforming growth factor beta receptor signaling pathway

Research Articles on ZYX

Similar Products

Product Notes

The ZYX zyx (Catalog #AAA9417312) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZYX Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZYX can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Western Blot: 1:200-1000 Immunohistochemistry: 1: 20-100. Researchers should empirically determine the suitability of the ZYX zyx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZYX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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