Western Blot (WB)
(Western Blot: Sample: Lane1: Human Jurkat Cells; Lane2: Human K-562 Cells.)
Rabbit anti-Human Asparagine Synthetase Polyclonal Antibody | anti-ASNS antibody
Polyclonal Antibody to Asparagine Synthetase (ASNS)
Immunocytochemistry in formalin fixed cells: 5-20ug/mL
Immunohistochemistry in formalin fixed frozen section: 5-20ug/mL
Immunohistochemistry in paraffin section: 5-20ug/mL
Enzyme-linked Immunosorbent Assay: 0.05-2ug/mL
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Jurkat Cells; Lane2: Human K-562 Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Jurkat Cells; Lane2: Human K-562 Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant ASNS, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant ASNS, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type K562 cell lysate; Lane 2: ASNS knockout K562 cell lysate; Predicted MW: 64,62,54kd Observed MW: 62kd Primary Ab: 3ug/ml Rabbit Anti-Human ASNS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type K562 cell lysate; Lane 2: ASNS knockout K562 cell lysate; Predicted MW: 64,62,54kd Observed MW: 62kd Primary Ab: 3ug/ml Rabbit Anti-Human ASNS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
Uniprot Description
ASNS: asparagine synthetase, an ubiquitous enzyme in mammalian organs, is involved in the first step of the synthesis of L-asparagine from L-glutamine and L-aspartate. Highly regulated in response to cell stress. Induced by dietary protein deficiency, imbalanced amino acid intake via the amino acid response, or ER stress via the EIF2AK3-eIF2-ATF4 branch of the unfolded protein response (UPR). Expression induced by nutrient deprivation or hypoxia in solid tumors. Essential for brain development but apparently not for that of other organs. Deficiency impairs brain development resulting in microcephaly and progressive cerebral atrophy, as well as deficiencies in learning and memory. The mechanism through which ASNS induces neuronal damage may be via damage induced by excessive excitability induced by elevated levels of aspartate/glutamate, via asparagine deficiency, or both. Three isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Cell cycle regulation; Cell development/differentiation; EC 6.3.5.4; Energy Metabolism - nitrogen; Ligase
Chromosomal Location of Human Ortholog: 7q21.3
Cellular Component: cytosol
Molecular Function: asparagine synthase (glutamine-hydrolyzing) activity; ATP binding; cofactor binding; protein binding; protein homodimerization activity
Biological Process: amino acid biosynthetic process; asparagine biosynthetic process; cellular response to glucose starvation; cellular response to hormone stimulus; glutamine metabolic process; liver development; negative regulation of apoptosis; positive regulation of mitotic cell cycle; response to amino acid; response to follicle-stimulating hormone stimulus; response to light stimulus; response to mechanical stimulus; response to methotrexate; response to toxin
Disease: Asparagine Synthetase Deficiency
Research Articles on ASNS
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Product Notes
The ASNS asns (Catalog #AAA2027582) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Asparagine Synthetase (ASNS) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Asparagine Synthetase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1-5ug/mL Immunocytochemistry in formalin fixed cells: 5-20ug/mL Immunohistochemistry in formalin fixed frozen section: 5-20ug/mL Immunohistochemistry in paraffin section: 5-20ug/mL Enzyme-linked Immunosorbent Assay: 0.05-2ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the ASNS asns for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Asparagine Synthetase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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