Rabbit anti-Human FRMPD4 Polyclonal Antibody | anti-KIAA0195 antibody

FRMPD4 Antibody

Cat. Num. AAA150540

• Gene Names: KIAA0195; TMEM94
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: FRMPD4 Antibody is affinity chromatography purified via peptide column.
• Synonyms: FRMPD4; Polyclonal Antibody; FRMPD4 Antibody; TMEM94; Uncharacterized protein KIAA0195; Transmembrane protein 94; KIAA0195; anti-KIAA0195 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: FRMPD4 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1356

• Applicable Applications for anti-KIAA0195 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: FRMPD4 antibody can be used for detection of FRMPD4 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: FRMPD4 antibody was raised against an 18 amino acid synthetic peptide from near the center of human FRMPD4.
• Buffer: FRMPD4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: FRMPD4 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of FRMPD4 in SK-N-SH cell lysate with FRMPD4 antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of FRMPD4 in human brain tissue with FRMPD4 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of FRMPD4 in human brain tissue with FRMPD4 antibody at 20 μg/mL.)

Related Product Information for anti-KIAA0195 antibody

FRMPD4 Antibody: The FERM and PDZ domain containing (FRMPD) protein family consists of four proteins that contain a FERM (Four-point-one, erzin, radixin, moesin) domain and at least one PDZ (PSD-95/Discs large/Zonula-occuldens-1) domain. FRMPD4, also known as Preso, also contains another protein interaction domain termed WW (domain with two conserved Trp residues) at its amino terminus. It was identified through a yeast two-hybrid screen using the PDZ domain of PSD-95 as bait and is highly expressed in multiple regions of the brain and is enriched in the postsynaptic density (PSD) fractions. Overexpression of FRMPD4 in cultured hippocampal neurons significantly increased the linear density of dendritic spines without changing their length and width; conversely, knockdown experiments using RNAi caused a decrease in spine density, indicating FRMPD4 positively regulates dendritic spine density but not morphology. The decreased level of FRMPD4 also resulted in reduced levels of excitatory synaptic transmission, suggesting that FRMPD4 is required for maintenance of excitatory synaptic transmission.

NCBI and Uniprot Product Information

• NCBI GI #: 157502197
• NCBI GeneID: 9772
• NCBI Accession #: NP_055553
• NCBI GenBank Nucleotide #: NM_014738.4
• UniProt Accession #: Q12767; O75536; Q86XF1
• Molecular Weight: 154,257 Da
• NCBI Official Full Name: uncharacterized protein KIAA0195
• NCBI Official Synonym Full Names: KIAA0195
• NCBI Official Symbol: KIAA0195
• NCBI Official Synonym Symbols: TMEM94
• NCBI Protein Information: uncharacterized protein KIAA0195; transmembrane protein 94
• UniProt Protein Name: Uncharacterized protein KIAA0195
• UniProt Gene Name: KIAA0195
• UniProt Synonym Gene Names: TMEM94
• UniProt Entry Name: K0195_HUMAN

Uniprot Description

KIAA0195: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: nucleoplasm; integral to membrane