Rabbit anti-Mouse Zeta Chain Associated Protein Kinase 70kDa (zAP70) Polyclonal Antibody | anti-zAP70 antibody

Polyclonal Antibody to Zeta Chain Associated Protein Kinase 70kDa (zAP70)

Cat. Num. AAA2005598

• Gene Names: Zap70; Srk; mur; mrtle; ZAP-70
• Reactivity: Mouse
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Zeta Chain Associated Protein Kinase 70kDa (zAP70); Polyclonal Antibody; Polyclonal Antibody to Zeta Chain Associated Protein Kinase 70kDa (zAP70); anti-zAP70 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against zAP70. It has been selected for its ability to recognize zAP70 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-A HPSTFTQPQR RVDTLNSDGY TPEPARLASS TDKPRPMPMD TSVYESPYSD PEELKDKKLF LKRENLLVAD IELGCGNFGS VRQGVYRMRK KQIDVAIKVL KQGTEKADKD EMMREAQIMH QLDNPYIVRL IGVCQAEALM LVMEMAGGGP LHKFLLGKKE EIPVSNVAEL LHQVAMGMKY LEEKNFVHRD LAARNVLLVN RHYAKISDFG LSKALGADDS YYTARSAGKW PLKWYAPECI NFRKFSSRSD VWSYGVTMWE AFSYGQKPYK KMKGPEVLDF IKQGKRMECP
• Sequence Length: 707

• Applicable Applications for anti-zAP70 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant zAP70 (Ala270~Pro560) expressed in E.coli.
• Cross Reactivity: Mouse
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2084478
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:MouseTissue))

NCBI and Uniprot Product Information

• NCBI GI #: 576067923
• NCBI GeneID: 22637
• NCBI Accession #: NP_001276541.1
• NCBI GenBank Nucleotide #: NM_001289612.1
• UniProt Accession #: P43404; P97455; Q80VV2; Q8CHJ3
• Molecular Weight: 35,255 Da
• NCBI Official Full Name: tyrosine-protein kinase ZAP-70 TZK
• NCBI Official Synonym Full Names: zeta-chain (TCR) associated protein kinase
• NCBI Official Symbol: Zap70
• NCBI Official Synonym Symbols: Srk; mur; mrtle; ZAP-70
• NCBI Protein Information: tyrosine-protein kinase ZAP-70
• UniProt Protein Name: Tyrosine-protein kinase ZAP-70
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Zap70
• UniProt Synonym Gene Names: Srk; Zap-70

NCBI Description

This gene encodes a member of the protein tyrosine kinase family. The encoded protein is essential for development of T lymphocytes and thymocytes, and functions in the initial step of T lymphocyte receptor-mediated signal transduction. A mutation in this gene causes chronic autoimmune arthritis, similar to rheumatoid arthritis in humans. Mice lacking this gene are deficient in alpha-beta T lymphocytes in the thymus. In humans, mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T lymphocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

ZAP70: a tyrosine kinase of the Syk family. Plays an essential role in regulation of the adaptive immune response. Phosphorylates two essential T cell adapter proteins: the linker for activation of T-cells (LAT) protein and LCP2. Regulates lymphocyte activation, motility, adhesion and cytokine expression of mature T-cells. Promotes survival and cell-cycle progression of developing double-negative thymocytes before positive selection. Associates with the T-cell antigen receptor (TCR) zeta-chain and is phosphorylated by Lck following antigen receptor activation. Required for internalization and degradation of the TCR complex through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Three alternatively spliced isoforms of the human protein have been described.

Protein type: EC 2.7.10.2; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Syk family; TK group

Chromosomal Location of Human Ortholog: 1 B|1 15.41 cM

Cellular Component: cytoplasm; cytosol; extrinsic component of cytoplasmic side of plasma membrane; immunological synapse; intercellular junction; membrane; membrane raft; plasma membrane; T cell receptor complex

Molecular Function: ATP binding; kinase activity; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; phosphotyrosine binding; protein binding; protein kinase activity; protein tyrosine kinase activity; receptor binding; transferase activity

Biological Process: adaptive immune response; beta selection; cell migration; immune response; immune system process; innate immune response; negative thymic T cell selection; peptidyl-tyrosine autophosphorylation; peptidyl-tyrosine phosphorylation; phosphorylation; positive regulation of alpha-beta T cell differentiation; positive regulation of alpha-beta T cell proliferation; positive regulation of calcium-mediated signaling; positive regulation of T cell differentiation; positive thymic T cell selection; protein amino acid phosphorylation; protein autophosphorylation; T cell receptor signaling pathway; thymic T cell selection; transmembrane receptor protein tyrosine kinase signaling pathway