Rabbit anti-Human, Mouse XPO1 Polyclonal Antibody | anti-XPO1 antibody

XPO1 Antibody

Cat. Num. AAA7049392

• Gene Names: XPO1; emb; CRM1; exp1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: XPO1; Polyclonal Antibody; XPO1 Antibody; Exportin-1; Exp1; Chromosome region maintenance 1 protein homolog; CRM1; anti-XPO1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 1071

• Applicable Applications for anti-XPO1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1: 500-1: 5000; IHC: 1: 20-1: 200; IF: 1: 50-1: 200
• Immunogen: Recombinant Human Exportin-1 protein (625-845AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Conjugation: Non-conjugated
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-5595 / sc-7825 / sc-7826
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: K562 whole cell lysate,NIH/3T3 whole cell lysateAll lanes: XPO1 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 124 KDaObserved band size: 124 KDa)

Immunohistochemistry (IHC)

(IHC image of MBS7049392 diluted at 1:150 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC)

(IHC image of MBS7049392 diluted at 1:150 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells using MBS7049392 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-XPO1 antibody

Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization.

NCBI and Uniprot Product Information

• NCBI GI #: 4507943
• NCBI GeneID: 7514
• NCBI Accession #: NP_003391.1
• NCBI GenBank Nucleotide #: NM_003400.3
• UniProt Accession #: O14980; Q63HP8; Q68CP3; Q99433; A6NL14; A8K1K5; D6W5E2
• Molecular Weight: 123,386 Da
• NCBI Official Full Name: exportin-1
• NCBI Official Synonym Full Names: exportin 1
• NCBI Official Symbol: XPO1
• NCBI Official Synonym Symbols: emb; CRM1; exp1
• NCBI Protein Information: exportin-1
• UniProt Protein Name: Exportin-1
• Protein Family: Exportin
• UniProt Gene Name: XPO1
• UniProt Synonym Gene Names: CRM1; Exp1
• UniProt Entry Name: XPO1_HUMAN

NCBI Description

This cell-cycle-regulated gene encodes a protein that mediates leucine-rich nuclear export signal (NES)-dependent protein transport. The protein specifically inhibits the nuclear export of Rev and U snRNAs. It is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. This protein also regulates NFAT and AP-1. [provided by RefSeq, Jan 2015]

Uniprot Description

Exportin-1: Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of an nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization. Belongs to the exportin family.

Protein type: Karyopherin; Nuclear export; Nuclear import; Nucleolus

Chromosomal Location of Human Ortholog: 2p15

Cellular Component: annulate lamellae; cytoplasm; cytosol; intracellular membrane-bound organelle; kinetochore; membrane; nuclear envelope; nuclear membrane; nucleolus; nucleoplasm; nucleus; ribonucleoprotein complex

Molecular Function: nuclear export signal receptor activity; nucleocytoplasmic transporter activity; protein binding; transporter activity

Biological Process: protein export from nucleus; regulation of mRNA stability; regulation of protein export from nucleus; ribosomal large subunit export from nucleus; ribosomal small subunit export from nucleus; ribosome export from nucleus; sister chromatid cohesion; viral reproduction