Rabbit anti-Human Exportin-1 Polyclonal Antibody | anti-XPO1 antibody

Exportin-1 (XPO1, Exp1, Chromosome Region Maintenance 1 Protein Homolog, CRM1)

Cat. Num. AAA649537

• Gene Names: XPO1; emb; CRM1; exp1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Exportin-1; Polyclonal Antibody; Exportin-1 (XPO1; Exp1; Chromosome Region Maintenance 1 Protein Homolog; CRM1); Anti -Exportin-1 (XPO1; anti-XPO1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human XPO1. Species sequence homology: Bovine, chimpanzee, canine, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

• Applicable Applications for anti-XPO1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1-3ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
• Immunogen: Synthetic peptide corresponding to aa390-408 from human XPO1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Spleen (formalin-fixed, paraffin-embedded) stained with XPO1 antibody at 10ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-XPO1 antibody

XPO1 mediates leucine-rich nuclear export signal (NES)-dependent protein transport. Exportin 1 specifically inhibits the nuclear export of Rev and U snRNAs. It is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. This protein also regulates NFAT and AP-1.

Product Categories/Family for anti-XPO1 antibody

Antibodies; Abs to RNA Processing

NCBI and Uniprot Product Information

• NCBI GI #: 4507943
• NCBI GeneID: 7514
• NCBI Accession #: NP_003391.1
• NCBI GenBank Nucleotide #: NM_003400.3
• UniProt Accession #: O14980; Q63HP8; Q68CP3; Q99433; A6NL14; A8K1K5; D6W5E2
• Molecular Weight: 123,386 Da
• NCBI Official Full Name: exportin-1
• NCBI Official Synonym Full Names: exportin 1 (CRM1 homolog, yeast)
• NCBI Official Symbol: XPO1
• NCBI Official Synonym Symbols: emb; CRM1; exp1
• NCBI Protein Information: exportin-1; CRM1, yeast, homolog; exportin 1 (CRM1, yeast, homolog); chromosome region maintenance 1 protein homolog; exportin-1 (required for chromosome region maintenance)
• UniProt Protein Name: Exportin-1
• Protein Family: Exportin
• UniProt Gene Name: XPO1
• UniProt Synonym Gene Names: CRM1; Exp1
• UniProt Entry Name: XPO1_HUMAN

NCBI Description

The protein encoded by this gene mediates leucine-rich nuclear export signal (NES)-dependent protein transport. Exportin 1 specifically inhibits the nuclear export of Rev and U snRNAs. It is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. This protein also regulates NFAT and AP-1. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization. Ref.9 Ref.10 Ref.11 Ref.23 Ref.25 Ref.34

Subunit structure: Found in a U snRNA export complex with RNUXA/PHAX, NCBP1, NCBP2, RAN, XPO1 and m7G-capped RNA

By similarity. Component of a nuclear export receptor complex composed of KPNB1, RAN, SNUPN and XPO1. Found in a trimeric export complex with SNUPN, RAN and XPO1. Found in a nuclear export complex with RANBP3 and RAN. Found in a 60S ribosomal subunit export complex with NMD3, RAN, XPO1. Interacts with DDX3X, NMD3, NUPL2, NUP88, NUP214, RANBP3 and TERT. Also found in complex with several viral proteins involved in RNAs' nuclear export like HIV-1 Rev and HTLV-1 Rex. Interacts presumably with influenza A nucleoprotein. Interacts with Epstein-Barr virus BMLF1. Interacts with NEMF (via its N-terminus). Interacts with the monomeric form of BIRC5/survivin deacetylated at 'Lys-129'. Interacts with DTNBP1 and SERTAD2; the interactions translocate DTNBP1 and SERTAD2 out of the nucleus. Interacts with ATF2. Ref.1 Ref.9 Ref.11 Ref.12 Ref.13 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24 Ref.26 Ref.27 Ref.29 Ref.33 Ref.34 Ref.37 Ref.38

Subcellular location: Cytoplasm. Nucleus › nucleoplasm. Nucleus › Cajal body. Nucleus › nucleolus. Note: Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm. Ref.1 Ref.2 Ref.25 Ref.26

Tissue specificity: Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney. Ref.1 Ref.2

Miscellaneous: Cellular target of leptomycin B (LMB), a XPO1/CRM1 nuclear export inhibitor.

Sequence similarities: Belongs to the exportin family.Contains 10 HEAT repeats.Contains 1 importin N-terminal domain.