Rabbit CRM1 Monoclonal Antibody | anti-XPO1 antibody

CRM1 Rabbit mAb

Cat. Num. AAA4752671

• Gene Names: XPO1; emb; CRM1; exp1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Affinity purified
• Synonyms: CRM1; Monoclonal Antibody; CRM1 Rabbit mAb; emb; exp1; anti-XPO1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Specificity: Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney.
• Purity/Purification: Affinity purified
• Form/Format: 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-XPO1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; ICC/IF: 1:100; FC/FACS: 1:50
• Immunogen: A synthetic peptide of human CRM1
• Conjugation: Unconjugated
• Modification: Unmodification
• Subcellular Location: Cytoplasm, Nucleus
• Preparation and Storage: Store at 4 degree C short term. Store at -20 degree C long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot detection of CRM1 in C6,Hela cell lysates using CRM1 Rabbit mAb(1:1000 diluted). Predicted band size:123kDa. Observed band size:123kDa.)

Related Product Information for anti-XPO1 antibody

This cell-cycle-regulated gene encodes a protein that mediates leucine-rich nuclear export signal (NES)-dependent protein transport. The protein specifically inhibits the nuclear export of Rev and U snRNAs. It is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. This protein also regulates NFAT and AP-1. [provided by RefSeq, Jan 2015]
Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization.

NCBI and Uniprot Product Information

• NCBI GI #: 68052989
• NCBI GeneID: 7514
• UniProt Accession #: O14980; Q63HP8; Q68CP3; Q99433; A6NL14; A8K1K5; D6W5E2
• Molecular Weight: 1071
• NCBI Official Full Name: Exportin-1
• NCBI Official Synonym Full Names: exportin 1
• NCBI Official Symbol: XPO1
• NCBI Official Synonym Symbols: emb; CRM1; exp1
• NCBI Protein Information: exportin-1; exportin 1 (CRM1 homolog, yeast); chromosome region maintenance 1 homolog; chromosome region maintenance 1 protein homolog; exportin-1 (required for chromosome region maintenance)
• UniProt Protein Name: Exportin-1
• Protein Family: Exportin
• UniProt Gene Name: XPO1
• UniProt Synonym Gene Names: CRM1; Exp1
• UniProt Entry Name: XPO1_HUMAN

NCBI Description

This cell-cycle-regulated gene encodes a protein that mediates leucine-rich nuclear export signal (NES)-dependent protein transport. The protein specifically inhibits the nuclear export of Rev and U snRNAs. It is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. This protein also regulates NFAT and AP-1. [provided by RefSeq, Jan 2015]

Uniprot Description

Exportin-1: Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of an nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization. Belongs to the exportin family.

Protein type: Karyopherin; Nuclear import; Nucleolus; Nuclear export

Chromosomal Location of Human Ortholog: 2p15

Cellular Component: kinetochore; nucleoplasm; Cajal body; nuclear membrane; intracellular membrane-bound organelle; membrane; cytoplasm; nucleolus; nuclear envelope; cytosol; ribonucleoprotein complex; nucleus; annulate lamellae

Molecular Function: protein domain specific binding; protein binding; RNA binding; transporter activity; Ran GTPase binding; protein transporter activity; nucleocytoplasmic transporter activity

Biological Process: response to drug; ribosomal small subunit export from nucleus; mRNA transport; viral reproduction; transforming growth factor beta receptor signaling pathway; regulation of protein catabolic process; gene expression; viral infectious cycle; negative regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; protein export from nucleus; negative regulation of transforming growth factor beta receptor signaling pathway; ribosomal large subunit export from nucleus; regulation of protein export from nucleus