Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AF6762) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Rabbit anti-Human, Mouse XIRP1 Polyclonal Antibody | anti-XIRP1 antibody
XIRP1 Antibody
Peptide ELISA: 1:20,000-1:40,000
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AF6762) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AF6762) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
NCBI and Uniprot Product Information
Uniprot Description
CMYA1: Protects actin filaments from depolymerization. Belongs to the Xin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Actin-binding; Cell adhesion; Cell development/differentiation
Chromosomal Location of Human Ortholog: 9|9 F4
Cellular Component: cell junction; cell-cell adherens junction; fascia adherens; filamentous actin; focal adhesion; intercalated disc; stress fiber
Molecular Function: actin binding; filamin binding; RNA binding
Biological Process: actin cytoskeleton organization; cardiac muscle cell development; heart development; heart morphogenesis; negative regulation of cell proliferation; negative regulation of protein binding; Notch signaling pathway; regulation of membrane potential; sarcomere organization
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Product Notes
The XIRP1 xirp1 (Catalog #AAA9613783) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The XIRP1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's XIRP1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA). IF/ICC: 1:100-1:500 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the XIRP1 xirp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "XIRP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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