Mouse P-Selectin ELISA Kit | SELP elisa kit

Mouse P-Selectin ELISA Kit

Cat. Num. AAA763762

• Gene Names: SELP; CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
• Reactivity: Mouse
• Synonyms: P-Selectin; Mouse P-Selectin ELISA Kit; P-Selectin/CD62P/GMP140/GRMP/PADGEM/PSEL/SELP/CD62P antigen/FLJ45155/PADGEMantigen CD62)/selectin P; SELP elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of SELP. No significant cross-reactivity or interference between SELP and analogues was observed.
• Sequence Length: 830

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SELP were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SELP were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SELP elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- SELP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- SELP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SELP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SELP can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 157419154
• NCBI GeneID: 6403
• NCBI Accession #: NP_002996.2
• NCBI GenBank Nucleotide #: NM_003005.3
• Molecular Weight: 90,834 Da
• NCBI Official Full Name: P-selectin
• NCBI Official Synonym Full Names: selectin P
• NCBI Official Symbol: SELP
• NCBI Official Synonym Symbols: CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
• NCBI Protein Information: P-selectin
• UniProt Protein Name: P-selectin
• Protein Family: P-selectin
• UniProt Gene Name: SELP
• UniProt Synonym Gene Names: GMRP; GRMP; GMP-140; LECAM3; PADGEM
• UniProt Entry Name: LYAM3_HUMAN

NCBI Description

This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. [provided by RefSeq, Jul 2008]

Uniprot Description

SELP: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the selectin/LECAM family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q22-q25

Cellular Component: cytoplasm; external side of plasma membrane; extracellular space; integral to plasma membrane; nucleoplasm; plasma membrane; platelet alpha granule membrane; platelet dense granule membrane

Molecular Function: calcium-dependent protein binding; fucose binding; glycoprotein binding; glycosphingolipid binding; heparin binding; lipopolysaccharide binding; protein binding; sialic acid binding

Biological Process: blood coagulation; cell adhesion; defense response to Gram-negative bacterium; heterophilic cell adhesion; inflammatory response; leukocyte adhesion; leukocyte migration; leukocyte tethering or rolling; platelet activation; platelet degranulation; positive regulation of cell adhesion; positive regulation of leukocyte migration; positive regulation of phosphoinositide 3-kinase cascade; regulation of cellular extravasation; regulation of integrin activation; response to lipopolysaccharide; response to organic cyclic substance

Disease: Ige Responsiveness, Atopic