Rabbit GFI1 Polyclonal Antibody | anti-GFI1 antibody

GFI1 Rabbit Polyclonal

Cat. Num. AAA769661

• Gene Names: GFI1; SCN2; GFI-1; GFI1A; ZNF163
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: GFI1; Polyclonal Antibody; GFI1 Rabbit Polyclonal; anti-GFI1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 422

• Applicable Applications for anti-GFI1 antibody: ELISA (EIA), Western Blot (WB)
• Immunogen: Growth factor independent 1 transcription repressor
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Western Blot (WB)

(K-562 cells were subjected to SDS PAGE followed by western blot with MBS769661 (GFI1 antibody) at dilution of 1:500)

NCBI and Uniprot Product Information

• NCBI GI #: 21618436
• NCBI GeneID: 2672
• Molecular Weight: 45,297 Da
• NCBI Official Full Name: GFI1 protein
• NCBI Official Synonym Full Names: growth factor independent 1 transcriptional repressor
• NCBI Official Symbol: GFI1
• NCBI Official Synonym Symbols: SCN2; GFI-1; GFI1A; ZNF163
• NCBI Protein Information: zinc finger protein Gfi-1
• UniProt Protein Name: Zinc finger protein Gfi-1
• Protein Family: Zinc finger protein
• UniProt Gene Name: GFI1
• UniProt Synonym Gene Names: ZNF163
• UniProt Entry Name: GFI1_HUMAN

NCBI Description

This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GFI1: Transcription repressor essential for hematopoiesis. Functions in a cell-context and development-specific manner. Binds to 5'-TAAATCAC[AT]GCA-3' in the promoter region of a large number of genes. Component of several complexes, including the EHMT2- GFI1-HDAC1, AJUBA-GFI1-HDAC1 and RCOR-GFI-KDM1A-HDAC complexes, that suppress, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Regulates neutrophil differentiation, promotes proliferation of lymphoid cells, and is required for granulocyte development. Mediates, together with U2AF1L4, the alternative splicing of CD45 and controls T-cell receptor signaling. Regulates the endotoxin- mediated Toll-like receptor (TLR) inflammatory response by antagonizing RELA. Cooperates with CBFA2T2 to regulate ITGB1- dependent neurite growth. Controls cell-cycle progression by repressing CDKNIA/p21 transcription in response to TGFB1 via recruitment of GFI1 by ZBTB17 to the CDKNIA/p21 and CDKNIB promoters. Required for the maintenance of inner ear hair cells. Defects in GFI1 are a cause of neutropenia severe congenital autosomal dominant type 2 (SCN2). SCN2 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Defects in GFI1 are a cause of dominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA). NI-CINA is a relatively mild form of neutropenia diagnosed in adults, but predisposing to leukemia in a subset of patients.

Protein type: C2H2-type zinc finger protein; Cell development/differentiation; Transcription factor; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: nuclear body; nuclear matrix; nucleus; transcriptional repressor complex

Molecular Function: metal ion binding; protein binding

Biological Process: G1/S-specific transcription in mitotic cell cycle; inhibition of NF-kappaB transcription factor; myeloid cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of histone H3-K4 methylation; regulation of toll-like receptor signaling pathway; transcription, DNA-dependent; viral reproduction

Disease: Neutropenia, Nonimmune Chronic Idiopathic, Of Adults; Neutropenia, Severe Congenital, 2, Autosomal Dominant