Rabbit WEE1 Polyclonal Antibody | anti-WEE1 antibody

WEE1 (Phospho-Ser642) Antibody

Cat. Num. AAA9407093

• Gene Names: WEE1; WEE1A; WEE1hu
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: WEE1; Polyclonal Antibody; WEE1 (Phospho-Ser642) Antibody; WEE1A; WEE1hu; kinase WEE1; anti-WEE1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of WEE1 only when phosphorylated at serine 642.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 432

• Applicable Applications for anti-WEE1 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:1000
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of Serine 642(S-V-S(p)-L-T) derived from Human WEE1.
• Modification: Phospho-Ser642
• Target Name: WEE1
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

(Western blot analysis of extracts from 293 cells treated with etoposide using WEE1 (Phospho-Ser642) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Related Product Information for anti-WEE1 antibody

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.Watanabe N., EMBO J. 14:1878-1891(1995).Cichutek A., Cytogenet. Cell Genet. 93:277-283(2001).Igarashi M., Nature 353:80-83(1991)

Product Categories/Family for anti-WEE1 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 221307502
• NCBI GeneID: 7465
• NCBI Accession #: NP_001137448.1
• NCBI GenBank Nucleotide #: NM_001143976.1
• UniProt Accession #: P30291; B3KVE1; D3DQV0
• Molecular Weight: 49,072 Da
• NCBI Official Full Name: wee1-like protein kinase isoform 2
• NCBI Official Synonym Full Names: WEE1 G2 checkpoint kinase
• NCBI Official Symbol: WEE1
• NCBI Official Synonym Symbols: WEE1A; WEE1hu
• NCBI Protein Information: wee1-like protein kinase
• UniProt Protein Name: Wee1-like protein kinase
• Protein Family: Wee1-like protein kinase
• UniProt Gene Name: WEE1
• UniProt Synonym Gene Names: WEE1hu
• UniProt Entry Name: WEE1_HUMAN

NCBI Description

This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]

Uniprot Description

Wee1: a protein kinase of the WEE family. A nuclear protein which catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

Protein type: Protein kinase, Other; EC 2.7.10.2; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; WEE family

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; magnesium ion binding; ATP binding

Biological Process: mitosis; peptidyl-tyrosine phosphorylation; establishment of cell polarity; regulation of cell cycle; cell division; microtubule cytoskeleton organization and biogenesis; mitotic cell cycle; blood coagulation; G2/M transition of mitotic cell cycle; neurite morphogenesis; G1/S transition of mitotic cell cycle