Western Blot (WB)
(Western blot analysis of anti-IDE Antibody (Center) in A375 cell line lysates (35ug/lane). IDE (arrow) was detected using the purified Pab.)
Rabbit IDE Polyclonal Antibody | anti-IDE antibody
IDE Antibody (Center)
Western Blot (WB)
(Western blot analysis of anti-IDE Antibody (Center) in A375 cell line lysates (35ug/lane). IDE (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-IDE Antibody (Center) in A375 cell line lysates (35ug/lane). IDE (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDE antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDE antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB)
(Western blot analysis of lysate from K562 cell line, using IDE Antibody (Center). MBS9212110 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Western Blot (WB)
(Western blot analysis of lysate from K562 cell line, using IDE Antibody (Center). MBS9212110 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Kim,M.,J. Biol. Chem. 282 (11), 7825-7832 (2007)
Radulescu,R.T.,Int. J. Oncol. 30 (1), 73-80 (2007)
Li,Q.,Cell 127 (2), 305-316 (2006)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009]
Uniprot Description
IDE: Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia. Belongs to the peptidase M16 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease; EC 3.4.24.56; Cell surface; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 10q23-q25
Cellular Component: nucleoplasm; peroxisomal matrix; extracellular space; cell surface; mitochondrion; cytoplasm; plasma membrane; peroxisome; nucleus; cytosol
Molecular Function: ubiquitin binding; protein homodimerization activity; zinc ion binding; metalloendopeptidase activity; beta-amyloid binding; ATPase activity; peptide binding; beta-endorphin binding; insulin binding; protein binding; glycoprotein binding; ATP binding; receptor binding
Biological Process: negative regulation of proteolysis; positive regulation of protein oligomerization; viral reproduction; proteolysis involved in cellular protein catabolic process; protein heterooligomerization; beta-amyloid metabolic process; insulin receptor signaling pathway; hormone catabolic process; determination of adult life span; proteolysis; protein homooligomerization; protein homotetramerization
Research Articles on IDE
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Product Notes
The IDE ide (Catalog #AAA9212110) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 406-435. The IDE Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's IDE can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the IDE ide for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IDE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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