Western Blot (WB)
(Western blot analysis of control and GDI-2 knockout HEK 293T cell lysate using Rabbit anti GDI-2 antibody at a 1/2000 dilution. 25 mug cell lysate was loaded in each lane, 3% nonfat dry milk in TBST was used for blocking)
Rabbit anti-Human GDI-2 Polyclonal Antibody | anti-GDI-2 antibody
Rabbit Anti GDI-2
Gene Names
GDI2; RABGDIB; HEL-S-46e
Reactivity
Human
Applications
Western Blot
Purity
Purified IgG was prepared from whole serum by affinity chromatography.
Synonyms
GDI-2; Polyclonal Antibody; Rabbit Anti GDI-2; GDI-2 antibody; anti-GDI-2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified IgG was prepared from whole serum by affinity chromatography.
Form/Format
Phosphate Buffered Saline
Sequence Length
445
Applicable Applications for anti-GDI-2 antibody
Western Blot (WB)
Application Notes
WB: 1:200-1:3000
Rabbit anti GDI-2 antibody detects a band of approximately 51 kDa in various cell and tissue lysates under reducing conditions.
Rabbit anti GDI-2 antibody detects a band of approximately 51 kDa in various cell and tissue lysates under reducing conditions.
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 296-445 of human GDI-2.
Preservative Stabilizers
0.02% Sodium Azide
50% Glycerol
50% Glycerol
Preparation and Storage
Store at -20 degree C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Western Blot (WB)
(Western blot analysis of control and GDI-2 knockout HEK 293T cell lysate using Rabbit anti GDI-2 antibody at a 1/2000 dilution. 25 mug cell lysate was loaded in each lane, 3% nonfat dry milk in TBST was used for blocking)
Western Blot (WB)
(Western blot analysis of control and GDI-2 knockout HEK 293T cell lysate using Rabbit anti GDI-2 antibody at a 1/2000 dilution. 25 mug cell lysate was loaded in each lane, 3% nonfat dry milk in TBST was used for blocking)
Related Product Information for anti-GDI-2 antibody
Rabbit anti GDI-2 antibody recognizes Rab GDP dissociation inhibitor beta, also known as Rab GDI beta, GDI-2, guanosine diphosphate dissociation inhibitor 2. GDI-2 is a member of a family of chaperone proteins, which control Rho GTPases (DerMardirossian and Bokoch 2005). It has been shown to function as a metastasis suppressor in bladder cancer (Gildea et al. 2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
rab GDP dissociation inhibitor beta isoform 1
NCBI Official Synonym Full Names
GDP dissociation inhibitor 2
NCBI Official Symbol
GDI2
NCBI Official Synonym Symbols
RABGDIB; HEL-S-46e
NCBI Protein Information
rab GDP dissociation inhibitor beta
UniProt Protein Name
Rab GDP dissociation inhibitor beta
UniProt Gene Name
GDI2
UniProt Synonym Gene Names
RABGDIB; Rab GDI beta; GDI-2
NCBI Description
GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI2 is ubiquitously expressed. The GDI2 gene contains many repetitive elements indicating that it may be prone to inversion/deletion rearrangements. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
Research Articles on GDI-2
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Product Notes
The GDI-2 gdi2 (Catalog #AAA225600) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti GDI-2 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GDI-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:200-1:3000 Rabbit anti GDI-2 antibody detects a band of approximately 51 kDa in various cell and tissue lysates under reducing conditions. Researchers should empirically determine the suitability of the GDI-2 gdi2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GDI-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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