Rabbit anti-Human Wee1 Polyclonal Antibody | anti-WEE1 antibody

Wee1 (S123) Antibody

Cat. Num. AAA9231084

• Gene Names: WEE1; WEE1A; WEE1hu
• Reactivity: Human
• Applications: Western Blot, ELISA
• Synonyms: Wee1; Polyclonal Antibody; Wee1 (S123) Antibody; Wee1-like protein kinase; WEE1hu; 2.7.10.2; Wee1A kinase; WEE1; anti-WEE1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This Wee1 (S123) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 96-130 amino acids from human Wee1.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 432

• Applicable Applications for anti-WEE1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:2000
• Antigen Type: Synthesized Peptide
• Antigen Source: Human
• Cellular Location: Nucleus.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-Wee1 (S123) Antibody at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: Jurkat whole cell lysateLane 3: K562 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 72 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-WEE1 antibody

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Product Categories/Family for anti-WEE1 antibody

Primary Antibodies; Cell Biology

References

Watanabe N.,et al.EMBO J. 14:1878-1891 (1995). Cichutek A.,et al.Cytogenet. Cell Genet. 93:277-283 (2001). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Taylor T.D.,et al.Nature 440:497-500 (2006). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 221307502
• NCBI GeneID: 7465
• NCBI Accession #: NP_001137448.1
• NCBI GenBank Nucleotide #: NM_001143976.1
• UniProt Accession #: P30291; B3KVE1; D3DQV0
• Molecular Weight: 71597 Da
• NCBI Official Full Name: wee1-like protein kinase isoform 2
• NCBI Official Synonym Full Names: WEE1 G2 checkpoint kinase
• NCBI Official Symbol: WEE1
• NCBI Official Synonym Symbols: WEE1A; WEE1hu
• NCBI Protein Information: wee1-like protein kinase
• UniProt Protein Name: Wee1-like protein kinase
• Protein Family: Wee1-like protein kinase
• UniProt Gene Name: WEE1
• UniProt Synonym Gene Names: WEE1hu

NCBI Description

This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]

Uniprot Description

Wee1: a protein kinase of the WEE family. A nuclear protein which catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

Protein type: EC 2.7.10.2; Kinase, protein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); WEE family

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: nucleolus; nucleoplasm; nucleus

Molecular Function: kinase activity; protein binding; protein-tyrosine kinase activity

Biological Process: G2/M transition of mitotic cell cycle; regulation of cell cycle