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Western Blot (WB)
(Anti-WDR70 Antibody (C-term) at 1:1000 dilution + HL-60 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 70 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit WDR70 Polyclonal Antibody | anti-WDR70 antibody
WDR70 Antibody (C-term)
Reactivity
HumanPredicted Reactivity: Bovine, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Synonyms
WDR70; Polyclonal Antibody; WDR70 Antibody (C-term); Affinity Purified Rabbit Polyclonal Antibody (Pab); WD repeat-containing protein 70; anti-WDR70 antibody
Host
Rabbit
Reactivity
Human
Predicted Reactivity: Bovine, Mouse, Rat
Predicted Reactivity: Bovine, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit Ig
Clone Number
RB29043
Specificity
This WDR70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 575-604 amino acids from the C-terminal region of human WDR70.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applicable Applications for anti-WDR70 antibody
Western Blot (WB), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
Application Notes
WB: 1:1000
FC: 1:10~50
IHC-P: 1:50~100
FC: 1:10~50
FC: 1:10~50
IHC-P: 1:50~100
FC: 1:10~50
Antigen Source
Human
Antigen Region
575-604
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Anti-WDR70 Antibody (C-term) at 1:1000 dilution + HL-60 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 70 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-WDR70 Antibody (C-term) at 1:1000 dilution + HL-60 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 70 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of WDR70 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of WDR70 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Western Blot (WB)
(WDR70 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the WDR70 antibody detected the WDR70 protein (arrow).)
Western Blot (WB)
(WDR70 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the WDR70 antibody detected the WDR70 protein (arrow).)
Immunohistochemistry (IHC)-Paraffin
(WDR70 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WDR70 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)-Paraffin
(WDR70 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WDR70 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(WDR70 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(WDR70 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-WDR70 antibody
References
Olsen, J.V., et al. Cell 127(3):635-648(2006)Ota, T., et al. Nat. Genet. 36(1):40-45(2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
73,201 Da
NCBI Official Full Name
WD repeat-containing protein 70
NCBI Official Synonym Full Names
WD repeat domain 70
NCBI Official Symbol
WDR70
NCBI Protein Information
WD repeat-containing protein 70
UniProt Protein Name
WD repeat-containing protein 70
Protein Family
UniProt Gene Name
WDR70
UniProt Entry Name
WDR70_HUMAN
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Product Notes
The WDR70 wdr70 (Catalog #AAA9232309) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WDR70 Antibody (C-term) reacts with Human Predicted Reactivity: Bovine, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's WDR70 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin. WB: 1:1000 FC: 1:10~50 IHC-P: 1:50~100 FC: 1:10~50. Researchers should empirically determine the suitability of the WDR70 wdr70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WDR70, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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