Western Blot (WB)
(The anti-FER Pab is used in Western blot to detect FER in HL-60 cell lysate.)
Rabbit anti-Human FER Polyclonal Antibody | anti-FER antibody
FER Antibody (C-term)
Western Blot (WB)
(The anti-FER Pab is used in Western blot to detect FER in HL-60 cell lysate.)
Western Blot (WB)
(The anti-FER Pab is used in Western blot to detect FER in HL-60 cell lysate.)
Immunohistochemistry (IHC)
(FER Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FER Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(FER Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FER Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of FER Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of FER Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Flow Cytometry (FC/FACS)
(FER Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(FER Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rosato, R., et al., Mol. Cell. Biol. 18(10):5762-5770 (1998).
Kim, L., et al., J. Biol. Chem. 273(36):23542-23548 (1998).
Warrington, J.A., et al., Genomics 11(3):701-708 (1991).
Morris, C., et al., Cytogenet. Cell Genet. 53(4):196-200 (1990).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the FPS/FES family of non-transmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome X. [provided by RefSeq, Apr 2015]
Uniprot Description
Fer: a non-receptor tyrosine kinase of the Fer family. Regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. Increased expression associated with prostate cancer proliferation, and antisense blocks soft agar growth.
Protein type: Oncoprotein; Kinase, protein; EC 2.7.10.2; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group; Fer family
Chromosomal Location of Human Ortholog: 5q21
Cellular Component: microtubule cytoskeleton; extrinsic to internal side of plasma membrane; lamellipodium; nuclear chromatin; cytoplasm; cell cortex; nucleus; cell junction; cytosol; actin cytoskeleton
Molecular Function: protein binding; cadherin binding; gamma-catenin binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; actin binding; lipid binding; protein phosphatase 1 binding; protein kinase binding; Rab GTPase binding; ATP binding; epidermal growth factor receptor binding
Biological Process: regulation of mast cell degranulation; regulation of protein amino acid phosphorylation; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; diapedesis; cytokine and chemokine mediated signaling pathway; tyrosine phosphorylation of Stat3 protein; response to lipopolysaccharide; negative regulation of mast cell activation during immune response; platelet-derived growth factor receptor signaling pathway; microtubule cytoskeleton organization and biogenesis; chemotaxis; protein amino acid phosphorylation; regulation of epidermal growth factor receptor signaling pathway; activation of NF-kappaB transcription factor; regulation of cell proliferation; cell proliferation; cellular response to insulin stimulus; positive regulation of actin filament polymerization; positive regulation of cell proliferation; actin cytoskeleton reorganization; innate immune response; mitotic cell cycle; cell differentiation; cell adhesion; positive regulation of cell migration
Research Articles on FER
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Product Notes
The FER fer (Catalog #AAA9213295) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 782-811. The FER Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FER can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the FER fer for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FER, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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