Rabbit anti-Human NOS2A Polyclonal Antibody | anti-NOS2 antibody

NOS2A Antibody (Center)

Cat. Num. AAA9214112

• Gene Names: NOS2; NOS; INOS; NOS2A; HEP-NOS
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: NOS2A; Polyclonal Antibody; NOS2A Antibody (Center); Nitric oxide synthase; inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2; NOS2; anti-NOS2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This NOS2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 830-860 amino acids from the Central region of human NOS2A.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 830-860
• Sequence Length: 1,114

• Applicable Applications for anti-NOS2 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-NOS2A Antibody (Center)at 1:2000 dilution + Y79 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 131 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Anti-NOS2A Antibody (Center)at 1:2000 dilution + A549 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 131 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(NOS2A Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NOS2A antibody detected the NOS2A protein (arrow).)

Immunohistochemistry (IHC)

(NOS2A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NOS2A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(NOS2A Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of NOS2A Antibody (Center) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-NOS2 antibody

Nitric oxide is a reactive free radical which acts as a
biologic mediator in several processes, including neurotransmission
and antimicrobial and antitumoral activities. This gene encodes a
nitric oxide synthase which is expressed in liver and is inducible
by a combination of lipopolysaccharide and certain cytokines. Three
related pseudogenes are located within the Smith-Magenis syndrome
region on chromosome 17.

Product Categories/Family for anti-NOS2 antibody

Cancer; Metabolism; Neuroscience; Signal transduction

References

Ryk, C., et al. J. Urol. 184(5):2150-2157(2010)
Planche, T., et al. Am. J. Physiol. Regul. Integr. Comp. Physiol. 299 (5), R1248-R1253 (2010) :
Feng, C., et al. FEBS Lett. 584(20):4335-4338(2010)
Mokrzycka, M., et al. Folia Histochem. Cytobiol. 48(2):191-196(2010)
Tupitsyna, T.V., et al. Mol. Gen. Mikrobiol. Virusol. 3, 3-7 (2010) :

NCBI and Uniprot Product Information

• NCBI GI #: 24041029
• NCBI GeneID: 4843
• NCBI Accession #: NP_000616.3
• NCBI GenBank Nucleotide #: NM_000625.4
• UniProt Accession #: P35228; O60757; O94994; Q16263; Q16692; Q4TTS5; Q9UD42; A1L3U5; B7ZLY2
• Molecular Weight: 131117
• NCBI Official Full Name: nitric oxide synthase, inducible
• NCBI Official Synonym Full Names: nitric oxide synthase 2, inducible
• NCBI Official Symbol: NOS2
• NCBI Official Synonym Symbols: NOS; INOS; NOS2A; HEP-NOS
• NCBI Protein Information: nitric oxide synthase, inducible
• UniProt Protein Name: Nitric oxide synthase, inducible
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: NOS2
• UniProt Synonym Gene Names: NOS2A; HEP-NOS; Inducible NOS; iNOS
• UniProt Entry Name: NOS2_HUMAN

NCBI Description

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

Uniprot Description

iNOS: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Homodimer. Binds SLC9A3R1. By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. Belongs to the NOS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - arginine and proline; EC 1.14.13.39; Oxidoreductase

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cortical cytoskeleton; perinuclear region of cytoplasm; cytoplasm; peroxisome; intracellular; nucleus; cytosol

Molecular Function: calmodulin binding; protein binding; protein homodimerization activity; FAD binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; FMN binding; iron ion binding; heme binding; NADP binding; receptor binding

Biological Process: circadian rhythm; interaction with host; superoxide metabolic process; positive regulation of guanylate cyclase activity; positive regulation of killing of cells of another organism; positive regulation of vasodilation; defense response to Gram-negative bacterium; regulation of cell proliferation; regulation of cellular respiration; positive regulation of leukocyte mediated cytotoxicity; innate immune response in mucosa; response to bacterium; defense response to bacterium; response to hypoxia; peptidyl-cysteine S-nitrosylation; arginine catabolic process; negative regulation of blood pressure; nitric oxide biosynthetic process; inflammatory response; negative regulation of protein catabolic process; blood coagulation; regulation of insulin secretion; nitric oxide mediated signal transduction

Disease: Hypertension, Essential; Malaria, Susceptibility To