Western Blot (WB)
(SPECC1 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).)
Rabbit SPECC1 Polyclonal Antibody | anti-SPECC1L antibody
SPECC1 Antibody (Center)
Western Blot (WB)
(SPECC1 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).)
Western Blot (WB)
(SPECC1 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).)
Western Blot (WB)
(SPECC1 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).)
Western Blot (WB)
(SPECC1 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).)
Immunohistochemistry (IHC)
(SPECC1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPECC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(SPECC1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPECC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(SPECC1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(SPECC1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of SPECC1 Antibody (Center) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of SPECC1 Antibody (Center) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a coiled-coil domain containing protein. The encoded protein may play a critical role in actin-cytoskeletal reorganization during facial morphogenesis. Mutations in this gene are a cause of oblique facial clefting-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A read-through transcript composed of SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and the downstream ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013]
Uniprot Description
SPECC1L: Involved in cytokinesis and spindle organization. May play a role in actin cytoskeleton organization and microtubule stabilization and hence required for proper cell adhesion and migration. Defects in SPECC1L are the cause of facial clefting oblique type 1 (OBLFC1). A rare form of facial clefting. A facial cleft is any of the fissures between the embryonic prominences that normally unite to form the face. Belongs to the cytospin-A family.
Protein type: Cell cycle regulation
Chromosomal Location of Human Ortholog: 22q11.23
Cellular Component: filamentous actin; cytoplasm; gap junction; microtubule organizing center; spindle
Biological Process: cell migration; negative regulation of microtubule depolymerization; cell division; negative regulation of actin filament depolymerization; cell cycle; actin cytoskeleton organization and biogenesis
Disease: Facial Clefting, Oblique, 1; Opitz Gbbb Syndrome, Type Ii
Research Articles on SPECC1L
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Product Notes
The SPECC1L specc1l (Catalog #AAA9210195) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 705-733. The SPECC1 Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Chicken, Zebrafish) and may cross-react with other species as described in the data sheet. AAA Biotech's SPECC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). WB~~1:1000. Researchers should empirically determine the suitability of the SPECC1L specc1l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPECC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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