Rabbit anti-Human WDR68 Polyclonal Antibody | anti-WDR68 antibody

WDR68, CT (DCAF7, HAN11, WDR68, DDB1-and CUL4-associated factor 7, WD repeat-containing protein 68, WD repeat-containing protein An11 homolog) (APC)

Cat. Num. AAA6363391

• Gene Names: DCAF7; AN11; HAN11; WDR68; SWAN-1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: WDR68; Polyclonal Antibody; CT (DCAF7; HAN11; DDB1-and CUL4-associated factor 7; WD repeat-containing protein 68; WD repeat-containing protein An11 homolog) (APC); DCAF7; WD repeat-containing protein An11 homolog; anti-WDR68 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-WDR68 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: WDR68 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-314 amino acids from the C-terminal region of human WDR68.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(WDR68 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the WDR68 antibody detected the WDR68 protein (arrow).)

Related Product Information for anti-WDR68 antibody

WDR68 is involved in craniofacial development. Acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches (By similarity). Associates with DIAPH1 and controls GLI1 transcriptional activity. Could be involved in normal and disease skin development. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.

Product Categories/Family for anti-WDR68 antibody

Antibodies; WD Repeat Proteins

References

Ritterhoff, S., et al. EMBO J. 29(22):3750-3761(2010). Gudbjartsson, D.F., et al. Nat. Genet. 40(5):609-615(2008). Morita, K., et al. J. Dermatol. Sci. 44(1):11-20(2006). Jin, J., et al. Mol. Cell 23(5):709-721(2006). Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 108936958
• NCBI GeneID: 10238
• NCBI Accession #: NP_005819.3
• NCBI GenBank Nucleotide #: NM_005828.4
• UniProt Accession #: P61962; O15491; Q9DAE4; B4E039; D3DU14
• Molecular Weight: 16,340 Da
• NCBI Official Full Name: DDB1- and CUL4-associated factor 7
• NCBI Official Synonym Full Names: DDB1 and CUL4 associated factor 7
• NCBI Official Symbol: DCAF7
• NCBI Official Synonym Symbols: AN11; HAN11; WDR68; SWAN-1
• NCBI Protein Information: DDB1- and CUL4-associated factor 7; WD repeat-containing protein 68; WD repeat-containing protein An11 homolog; human anthocyanin; seven-WD-repeat protein of the AN11 family-1
• UniProt Protein Name: DDB1- and CUL4-associated factor 7
• Protein Family: DDB1- and CUL4-associated factor
• UniProt Gene Name: DCAF7
• UniProt Synonym Gene Names: HAN11; WDR68
• UniProt Entry Name: DCAF7_HUMAN

NCBI Description

This gene encodes a protein with multiple WD40 repeats which facilitate protein-protein interactions and thereby enable the assembly of multiprotein complexes. This protein has been shown to function as a scaffold protein for protein complexes involved in kinase signaling. This highly conserved gene is present in eukaryotic plants, fungi, and animals. The ortholog of this gene was first identified in plants as a key regulator of anthocyanin biosynthesis and flower pigmentation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

WDR68: a WD40 repeat protein. WD40 repeats are found in a number of eukaryotic proteins that coordinate multi-protein complex assemblies. WD40 proteins are implicated in many functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: nucleoplasm; nuclear matrix; protein complex; cytoplasm

Molecular Function: protein binding

Biological Process: multicellular organismal development; protein ubiquitination