Porcine CD30 Ligand ELISA Kit | CD30L elisa kit

Porcine CD30 Ligand ELISA Kit

Cat. Num. AAA739488

• Gene Names: TNFSF8; CD153; CD30L; CD30LG
• Reactivity: Porcine
• Synonyms: CD30 Ligand; Porcine CD30 Ligand ELISA Kit; CD30L elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This CD30L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine CD30L. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD30L elisa kit

Principle of the Assay: CD30L ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD30L antibody and an CD30L-HRP conjugate. The assay sample and buffer are incubated together with CD30L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD30L concentration since CD30L from samples and CD30L-HRP conjugate compete for the anti-CD30L antibody binding site. Since the number of sites is limited, as more sites are occupied by CD30L from the sample, fewer sites are left to bind CD30L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD30L concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CD30L elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 2815515
• NCBI GeneID: 944
• Molecular Weight: 26,017 Da
• NCBI Official Full Name: CD30L protein
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 8
• NCBI Official Symbol: TNFSF8
• NCBI Official Synonym Symbols: CD153; CD30L; CD30LG
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 8; CD30-L; CD30 ligand; CD153 antigen; CD30 antigen ligand
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 8
• UniProt Gene Name: TNFSF8
• UniProt Synonym Gene Names: CD30L; CD30LG; CD30-L
• UniProt Entry Name: TNFL8_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

TNFSF8: Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells. Belongs to the tumor necrosis factor family.

Protein type: Apoptosis; Membrane protein, integral; Cytokine

Chromosomal Location of Human Ortholog: 9q33

Cellular Component: extracellular space; integral to plasma membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: cell proliferation; CD8-positive, alpha-beta T cell differentiation; defense response to Gram-positive bacterium; cell-cell signaling; apoptosis; positive regulation of transcription from RNA polymerase II promoter; immune response; signal transduction