Immunofluorescence (IF)
(MBS9605797 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human PPP1R14D Polyclonal Antibody | anti-PPP1R14D antibody
PPP1R14D Antibody
Gene Names
PPP1R14D; GBPI1; GBPI-1; CPI17-like
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PPP1R14D; Polyclonal Antibody; PPP1R14D Antibody; CPI17-like; Gastrointestinal and brain-specific PP1-inhibitory protein 1; GBPI; GBPI-1; GBPI1; Gut and brain phosphatase inhibitor 1; PKC dependent PP1 inhibitory protein; PP14D_HUMAN; Ppp1r14d; Protein phosphatase 1 regulatory subunit 14D; Protein phosphatase 1; regulatory (inhibitor) subunit 14D; anti-PPP1R14D antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
PPP1R14D antibody detects endogenous levels of total PPP1R14D
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
145
Applicable Applications for anti-PPP1R14D antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm.
Tissue Specificity
Detected in colon, intestine, kidney and brain cortex.
Predicted Cross Reactivity
Bovine, Horse, Sheep, Dog
Similarity
Bovine (82%), Horse (91%), Sheep (82%), Dog (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9605797 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9605797 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from COLO cells using PPP1R14D antibody.)
Western Blot (WB)
(Western blot analysis of extracts from COLO cells using PPP1R14D antibody.)
Related Product Information for anti-PPP1R14D antibody
Function: Inhibitor of PPP1CA. Has inhibitory activity only when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
Post-translational Modifications: Phosphorylated on several residues.
Similarity: Belongs to the PP1 inhibitor family.
Post-translational Modifications: Phosphorylated on several residues.
Similarity: Belongs to the PP1 inhibitor family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 24 kD
Predicted: 17 kDa
Predicted: 17 kDa
NCBI Official Full Name
protein phosphatase 1 regulatory subunit 14D isoform 1
NCBI Official Synonym Full Names
protein phosphatase 1 regulatory inhibitor subunit 14D
NCBI Official Symbol
PPP1R14D
NCBI Official Synonym Symbols
GBPI1; GBPI-1; CPI17-like
NCBI Protein Information
protein phosphatase 1 regulatory subunit 14D
UniProt Protein Name
Protein phosphatase 1 regulatory subunit 14D
Protein Family
UniProt Gene Name
PPP1R14D
UniProt Synonym Gene Names
GBPI; GBPI-1
NCBI Description
Protein phosphatase-1 (PP1; see MIM 176875) is a major cellular phosphatase that reverses serine/threonine protein phosphorylation. PPP1R14D is a PP1 inhibitor that itself is regulated by phosphorylation (Liu et al., 2004 [PubMed 12974676]).[supplied by OMIM, Feb 2010]
Uniprot Description
Inhibitor of PPP1CA. Has inhibitory activity only when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
Research Articles on PPP1R14D
Similar Products
Product Notes
The PPP1R14D ppp1r14d (Catalog #AAA9605797) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPP1R14D Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPP1R14D can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the PPP1R14D ppp1r14d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP1R14D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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