Rabbit anti-Human WDR12 Polyclonal Antibody | anti-WDR12 antibody

Anti-WDR12 Antibody, Rabbit Polyclonal

Cat. Num. AAA8119150

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: WDR12; Polyclonal Antibody; Anti-WDR12 Antibody; Rabbit Polyclonal; WDR12 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-YTM1 Antibody; WD repeat domain 12; anti-WDR12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human WDR12
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid. PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-WDR12 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human WDR12 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human WDR12 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Western Blot (WB)

(Anti-WDR12 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:48 kDa Observed band size:48 kDa)

Immunoprecipitation (IP)

(WDR12 was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate Lane B:0.5 mg HeLa Whole Cell Lysate Lane C:0.5 mg 293T Whole Cell Lysate Lane D:0.5 mg NIH3T3 Whole Cell Lysate 4 uL anti- WDR12 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti- WDR12 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 48 kDa Observed band size :48 kDa)

Related Product Information for anti-WDR12 antibody

Background: WDR12 (WD repeat protein 12), a member of the WD repeats family, plays a crucial role in the ribosome biogenesis pathway. WDR12 processes the 32S precursor rRNA but cannot affect the synthesis of the 45S/47S primary transcript. The mammalian PeBoW, a trimeric complex consisting of pescadillo (Pes1), block of proliferation (Bop1), and the WD-repeat protein 12 (WDR12), is essential for the processing and maturation of mammalian 5.8S and 28S ribosomal RNAs. The nucleolar protein pescadillo (PES) controls biogenesis of the 60S ribosomal subunit through functional interactions with Block of Proliferation 1 (BOP1) and WD Repeat Domain 12 (WDR12) in plants. WDR12 contributes to hepatocellular carcinoma (HCC) propagation, and that suppression of WDR12 may be a potential strategy for human HCC treatment.

References

Romes EM, et al. (2016) The crystal structure of the ubiquitin-like domain of ribosome assembly factor ytm1 and characterization of its interaction with the aaa-atpase midasin. J Biol Chem 291 (2): 882-893.Li JL, et al. (2020) Integrative genomic analyses identify wdr12 as a novel oncogene involved in glioblastoma. J Cell Physiol 235 (10): 7344-7355.Kellner M, et al. (2015) Dead-box helicase ddx27 regulates 3' end formation of ribosomal 47s rna and stably associates with the pebow-complex. Exp Cell Res 334 (1): 146-159.Ahn CS, et al. (2016) Functional characterization of the ribosome biogenesis factors pes, bop1, and wdr12 (pebow), and mechanisms of defective cell growth and proliferation caused by pebow deficiency in arabidopsis. J Exp Bot 67 (17): 5217-5232.