Rabbit VPS33A Polyclonal Antibody | anti-VPS33A antibody

VPS33A Antibody

Cat. Num. AAA8527179

• Gene Names: VPS33A; MPSPS
• Reactivity: Human; Predicted: Mouse, Rat
• Applications: ELISA, Western Blot
• Synonyms: VPS33A; Polyclonal Antibody; VPS33A Antibody; anti-VPS33A antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Mouse, Rat
• Clonality: Polyclonal
• Form/Format: Purified by protein affinity column containing 1% BSA and 0.02% NaN3
• Sequence Length: 596

• Applicable Applications for anti-VPS33A antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:20,000-1:80,000; WB: 1:200-1:1,000
• Preparation and Storage: Already reconstituted in 200ul sterile H2O. The antibody is stable for at least one year when stored at-20 degree C after reconstitution. Avoid repeated freeze/defrost cycles.

Testing Data

Related Product Information for anti-VPS33A antibody

Rabbit polyclonal VPS33A (1) antibody was raised against a recombinate human VPS33A protein 374-582aa (BC016617).

References

(1) Huang, Z.M., Chinen, M., Chang, P.J., et al. Targeting protein-trafficking pathways alters melanoma treatment sensitivity. Proc. Natl. Acad. Sci. U.S.A. 109 (2), 553-558 (2012).

NCBI and Uniprot Product Information

• NCBI GI #: 18105056
• NCBI GeneID: 65082
• NCBI Accession #: NP_075067.2
• NCBI GenBank Nucleotide #: NM_022916.5
• UniProt Accession #: Q96AX1; Q547V4; Q9H5Q0
• Molecular Weight: 67,611 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 33A isoform 1
• NCBI Official Synonym Full Names: VPS33A, CORVET/HOPS core subunit
• NCBI Official Symbol: VPS33A
• NCBI Official Synonym Symbols: MPSPS
• NCBI Protein Information: vacuolar protein sorting-associated protein 33A
• UniProt Protein Name: Vacuolar protein sorting-associated protein 33A
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS33A
• UniProt Synonym Gene Names: hVPS33A

NCBI Description

This gene encodes a tethering protein and a core subunit of the homotypic fusion and protein sorting (HOPS) complex. The HOPS complex and a second endosomal tethering complex called the class C core vacuole/endosome tethering (CORVET) complex, perform diverse functions in endocytosis including membrane tethering, RabGTPase interaction, activation and proofreading of synaptic-soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) assembly to drive membrane fusion, and endosome-to-cytoskeleton attachment. The HOPS complex controls endosome maturation as well as endosome traffic to the lysosome. This complex is essential for vacuolar fusion and is required for adaptor protein complex 3-dependent transport from the golgi to the vacuole. The encoded protein belongs to the Sec1/Munc18 (SM) family of SNARE-mediated membrane fusion regulators. Naturally occurring mutations in this gene are associated with a novel mucopolysaccharidosis-like disease. [provided by RefSeq, Apr 2017]

Uniprot Description

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed:23351085, PubMed:24554770, PubMed:25266290, PubMed:25783203). Required for fusion of endosomes and autophagosomes with lysosomes; the function is dependent on its association with VPS16 but not VIPAS39 (PubMed:25783203). The function in autophagosome-lysosome fusion implicates STX17 but not UVRAG (PubMed:24554770).